CLIA Kit for Growth Differentiation Factor 5 (GDF5) Rattus norvegicus (Rat) Sandwich CLIA

CDMP1; LAP4; SYNS2; BMP14; Radotermin; Cartilage-Derived Morphogenetic Protein-1; Bone morphogenetic protein 14; Lipopolysaccharide-associated protein 4

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  • CLIA Kit for Growth Differentiation Factor 5 (GDF5) Packages (Simulation)
  • CLIA Kit for Growth Differentiation Factor 5 (GDF5) Packages (Simulation)
  • CLIA Kit for Growth Differentiation Factor 5 (GDF5) Results demonstration
  • SCC110Ra.jpg Typical Standard Curve
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Recovery

Matrices listed below were spiked with certain level of recombinant Growth Differentiation Factor 5 (GDF5) and the recovery rates were calculated by comparing the measured value to the expected amount of Growth Differentiation Factor 5 (GDF5) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 87-102 94
EDTA plasma(n=5) 95-103 99
heparin plasma(n=5) 99-105 102

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Growth Differentiation Factor 5 (GDF5) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Growth Differentiation Factor 5 (GDF5) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Growth Differentiation Factor 5 (GDF5) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 81-104% 89-96% 96-105% 96-103%
EDTA plasma(n=5) 79-104% 78-105% 95-105% 85-93%
heparin plasma(n=5) 91-105% 96-104% 99-105% 93-101%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
Substrate A 1×10mL Substrate B 1×2mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 100µL Substrate Solution. Incubate 10 minutes at 37°C;
8. Read RLU value immediately.

CLIA Kit for Growth Differentiation Factor 5 (GDF5)

Test principle

The microplate provided in this kit has been pre-coated with an antibody specific to Growth Differentiation Factor 5 (GDF5). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Growth Differentiation Factor 5 (GDF5). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Growth Differentiation Factor 5 (GDF5) level in the sample or standard.;

Citations

  • ZrO2 surface chemically coated with hyaluronic acid hydrogel loading GDF-5 for osteogenesis in dentistryPubMed: 23218279
  • Photo-cured hyaluronic acid-based hydrogels containing growth and differentiation factor 5 (GDF-5) for bone tissue regenerationScienceDirect: S875632821300481X
  • In Vitro Osteogenic Differentiation Enhanced by Zirconia Coated with Nano-Layered Growth and Differentiation Factor-5Pubmed:27398455
  • Osteoblastic Differentiation of Functionalized Biphasic Hydroxyapatite and b-Tricalcium Phosphate with Recombinant Human Growth and Differentiation (rhGDF-5)
  • 3D Biomimetic Scaffold for Growth Factor Controlled Delivery: An In-Vitro Study of Tenogenic Events on Wharton's Jelly Mesenchymal Stem Cells34575523

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