CLIA Kit for Interferon Alpha (IFNa) Bos taurus; Bovine (Cattle) Sandwich CLIA

IFNA1; IFL; LeIF D; IFN; IFN-Alpha; IFNA13; IFN-A; IFNAP22; IFN-Alpha 1b; Interferon Alpha 1b; Interferon, Leukocytic; IFN, Leukocyte; Interferon alpha-D

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  • CLIA Kit for Interferon Alpha (IFNa) Packages (Simulation)
  • CLIA Kit for Interferon Alpha (IFNa) Packages (Simulation)
  • CLIA Kit for Interferon Alpha (IFNa) Results demonstration
  • SCA033Bo.jpg Typical Standard Curve
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Recovery

Matrices listed below were spiked with certain level of recombinant Interferon Alpha (IFNa) and the recovery rates were calculated by comparing the measured value to the expected amount of Interferon Alpha (IFNa) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 99-105 102
EDTA plasma(n=5) 96-104 99
heparin plasma(n=5) 85-101 88

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interferon Alpha (IFNa) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interferon Alpha (IFNa) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Interferon Alpha (IFNa) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 78-104% 79-96% 81-96% 97-105%
EDTA plasma(n=5) 85-99% 93-102% 80-97% 93-101%
heparin plasma(n=5) 78-90% 96-103% 95-102% 83-101%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
Substrate A 1×10mL Substrate B 1×2mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 100µL Substrate Solution. Incubate 10 minutes at 37°C;
8. Read RLU value immediately.

CLIA Kit for Interferon Alpha (IFNa)

Test principle

The microplate provided in this kit has been pre-coated with an antibody specific to Interferon Alpha (IFNa). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Interferon Alpha (IFNa). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Interferon Alpha (IFNa) level in the sample or standard.;

Citations

  • Tryptophan Stimulates Immune Response in Broiler Chickens Challenged with Infectious Bursal Disease VaccineMedwell: 2010610616
  • Immunostimulatory Effects of Arginine in Broiler Chickens Challenged with Vaccine Strain of Infectious Bursal Disease VirusMedwell: 2010594600
  • The cathelicidins LL-37 and rCRAMP are associated with pathogenic events of arthritis in humans and ratsPubMed: 23172753
  • Efficient intracellular delivery of siRNA with a safe multitargeted lipid-based nanoplatformPubMed: 23394132
  • Experimental rat model for alcohol-induced osteonecrosis of the femoral headPubMed: PMC3781777
  • A recombinant adenovirus bicistronically expressing porcine interferon-α and interferon-γ enhances antiviral effects against foot-and-mouth disease virusScienceDirect: S0166354214000266
  • Andrographolide exerts anti-hepatitis C virus activity by up-regulating haeme oxygenase-1 via the p38 MAPK/Nrf2 pathway in human hepatoma cellsOnlinelibrary: bph.12440
  • High efficiency of alphaviral gene transfer in combination with 5-fluorouracil in a mouse mammary tumor modelPubmed:24950740
  • Respiratory Mechanics and Plasma Levels of Tumor Necrosis Factor Alpha and Interleukin 6 Are Affected by Gas Humidification during Mechanical Ventilation in DogsPubmed:25036811
  • Infection of equine monocyte-derived macrophages with an attenuated equine infectious anemia virus (EIAV) strain induces a strong resistance to the infection by a virulent EIAV strainPubmed:25106750
  • Effect of SecinH3 on lung injury induced by sepsis of ratsPubMed: 26706678
  • siRNA Targeting the 2A pro Genomic Region Prevents Enterovirus 71 Replication In VitroPubmed:26886455
  • Sulforaphane Suppresses Hepatitis C Virus Replication by Up-ReCavia (Guinea pig )lating Heme Oxygenase-1 Expressionthrough PI3K/Nrf2 Pathway.Pubmed:27023634
  • Vaccination with recombinant Mycobacterium tuberculosis PknD attenuates bacterial dissemination to the brain in Cavia (Guinea pig )inea pigs.Pubmed:23776655
  • Impact of porcine reproductive and respiratory syndrome virus and porcine circovirus-2 infection on the potency of the classical swine fever vaccine (LOM strain).pubmed:27599928
  • Host miR-146a promotes replication of human cytomegalovirus by suppressing type I IFN response in MRC-5 cellsfiles:ijcep0036479.pdf
  • Serum Proteomics Analysis in Rats of Immunosuppression Induced by Chronic Stress.pubmed:27384769
  • Deoxyschizandrin suppresses dss-induced ulcerative colitis in mice.pubmed:27976641
  • MicroRNA-548j inhibits type I interferon production by targeting ZBTB11 in patients with chronic hepatitis Bpubmed:28342861
  • The down-regulation of casein kinase 1 alpha as a host defense response against infectious bursal disease virus infectionpubmed:28988058
  • Coinjection of a vaccine and anti-viral agents can provide fast-acting protection from foot-and-mouth diseasepubmed:28454913
  • Anti-high mobility group box 1 antibody ameliorates albuminuria in MRL/lprlupus-prone micepubmed:28649578
  • Fucoidan from Fucus vesiculosus suppresses hepatitis B virus replication by enhancing extracellular signal-regulated Kinase activation.pubmed:28915824
  • Toll-like receptor 9 ligands increase type I interferon induced B-cell activating factor expression in chronic rhinosinusitis with nasal polyposisPubmed:30056130
  • Role of Toll‐like receptor 9 signaling on activation of nasal polyp–derived fibroblasts and its association with nasal polypogenesisPubmed:29901280
  • Collagen triple helix repeat containing-1: a novel biomarker associated with disease activity in Systemic lupus erythematosusPubmed: 30336754
  • Role of melatonin in sleep deprivation‐induced intestinal barrier dysfunction in micePubmed: 30929267
  • Differential expression of type I interferon mRNA and protein levels induced by virulent Marek's disease virus infection in chickens
  • Adenovirus-mediated down-regulation of miR-21-5p alleviates experimental autoimmune uveoretinitis in micePubmed: 31228813
  • Scavenger receptor A impairs interferon response to HBV infection by limiting TRAF 3 ubiquitination through recruiting OTUB 1
  • Pathogenicity of porcine reproductive and respiratory syndrome virus (ORF5 RFLP 1–7–4 viruses) in ChinaPubmed: 32187856
  • A flagellin-adjuvanted inactivated porcine epidemic diarrhea virus (PEDV) vaccine provides enhanced immune protection against PEDV challenge in pigletsPubmed: 32253616
  • LDHB inhibition induces mitophagy and facilitates the progression of CSFV infectionPubmed: 32924761
  • Porcine soluble CD83 alleviates LPS-induced abortion in mice by promoting Th2 cytokine production, Treg cell generation and trophoblast invasionPubmed: 32810792
  • BNIP3 deletion ameliorated enterovirus 71 infection-induced hand, foot and mouth disease via inhibiting apoptosis, autophagy, and inflammation in micePubmed: 32717566
  • Aquaporin‑4 deletion ameliorates enterovirus 71 infection in micePubmed: 32582978
  • Determination of antiviral action of Long Non-coding RNA loc107051710 During Infectious Bursal Disease Virus Infection due to Enhancement of Interferon …
  • ГОРМОНАЛЬНЫЙ И ЦИТОКИНОВЫЙ ПРОФИЛЬ КРОВИ МОЛОЧНЫХ КОРОВ В РАННИЙ ПЕРИОД ГЕСТАЦИИ
  • Influenza virus neuraminidase regulates host CD8+ T-cell response in mice33293641
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  • Orexin A Suppresses the Expression of Exosomal PD-L1 in Colon Cancer and Promotes T Cell Activity by Inhibiting JAK2/STAT3 Signaling Pathway34097168
  • Clinical findings of Talaromyces marneffei infection among patients with anti-interferon-¦Ã immunodeficiency: a prospective cohort study34144700
  • Chronic developmental exposure to low-dose ([C8mim][PF6]) induces neurotoxicity and behavioural abnormalities in rats34555716
  • The anti-inflammatory activity of specific-sized hyaluronic acid oligosaccharides34823813
  • Effects of different selenium sources on duodenum and jejunum tight junction network and growth performance of broilers in a model of fluorine-induced …Pubmed:35066382
  • Self-assembled BPIV3 nanoparticles can induce comprehensive immune responses and protection against BPIV3 challenge by inducing dendritic cell maturation in …Pubmed:35395543

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