CLIA Kit for Interleukin 10 (IL10) Homo sapiens (Human) Sandwich CLIA

CSIF; IL10A; TGIF; Cytokine Synthesis Inhibitory Factor

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  • CLIA Kit for Interleukin 10 (IL10) Packages (Simulation)
  • CLIA Kit for Interleukin 10 (IL10) Packages (Simulation)
  • CLIA Kit for Interleukin 10 (IL10) Results demonstration
  • SCA056Hu.jpg Typical Standard Curve
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Recovery

Matrices listed below were spiked with certain level of recombinant Interleukin 10 (IL10) and the recovery rates were calculated by comparing the measured value to the expected amount of Interleukin 10 (IL10) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 92-99 96
EDTA plasma(n=5) 79-104 82
heparin plasma(n=5) 82-98 90

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interleukin 10 (IL10) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interleukin 10 (IL10) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Interleukin 10 (IL10) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 99-105% 97-105% 97-105% 90-99%
EDTA plasma(n=5) 82-93% 87-94% 89-97% 86-97%
heparin plasma(n=5) 95-103% 86-95% 89-97% 98-105%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
Substrate A 1×10mL Substrate B 1×2mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 100µL Substrate Solution. Incubate 10 minutes at 37°C;
8. Read RLU value immediately.

CLIA Kit for Interleukin 10 (IL10)

Test principle

The microplate provided in this kit has been pre-coated with an antibody specific to Interleukin 10 (IL10). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Interleukin 10 (IL10). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Interleukin 10 (IL10) level in the sample or standard.;

Citations

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  • A Two-Week Quercetin Supplementation in Horses Results in Moderate Accumulation of Plasma Flavonol ConcentrationsScience: Article
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  • A Systems Pharmacology Approach to Determine Active Compounds and Action Mechanisms of Xipayi KuiJie’an enema for Treatment of Ulcerative colitisdoi:10.1038/s41598-017-01335-w
  • The effect of feed supplementation with a copper-glycine chelate and copper sulphate on selected humoral and cell-mediated immune parameters, plasma superoxide dismutase activity, ceruloplasmin and cytokine concentration in broiler chickensdoi:10.1111
  • Tumor-associated macrophages promote tumor metastasis via the TGF-β/SOX9 axis in non-small cell lung cancerpubmed:29245941
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  • Oral administration of Clostridium butyricum CGMCC0313-1 reduces ovalbumin-induced allergic airway inflammation in micepubmed:28122397
  • Milk and serum IL-4, IL-6, IL-10, and amyloid A concentrations in cows with subclinical mastitis caused by coagulase-negative staphylococcipubmed:28964518
  • Alteration in some pro and anti-inflammatory cytokines associated with complete and incomplete gestation cycle of cows10.1080/09291016.2017.1319636
  • Role of 3-acetyl-11-keto-beta-boswellic acid in counteracting LPS-induced neuroinflammation via modulation of miRNA-155pubmed:29079998
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  • The immune impact of mimic endoscopic retrograde appendicitis therapy and appendectomy on rabbits of acute appendicitis.pubmed:29029533
  • Therapeutic effect of bone marrow mesenchymal stem cells pretreated with acetylsalicylic acid on experimental periodontitis in ratspubmed:29195233
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  • Association Between Endothelial Progenitor Cells and Treatment Response in Non-Squamous Non-small Cell Lung Cancer Treated with Bevacizumab.pubmed:28982871
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  • EGCG Maintains Th1/Th2 Balance and Mitigates Ulcerative Colitis Induced by Dextran Sulfate Sodium through TLR4/MyD88/NF-κB Signaling Pathway in Rats10.1155/2017/3057268
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  • Targeting central β2 receptors ameliorates streptozotocin-induced neuroinflammation via inhibition of glycogen synthase kinase3 pathway in micePubmed:29782959
  • Antioxidant, anti-inflammatory and synergistic anti-hyperglycemic effects of Malaysian propolis and metformin in streptozotocin–induced diabetic ratsPubmed:30026088
  • Resolution of bleomycin-induced murine pulmonary fibrosis via a splenic lymphocyte subpopulationPubmed:29690905
  • Influence of Vitamin D Deficiency on Progression of Experimental Otitis Media in RatsPubmed:29947185
  • Chitosan oligosaccharide ameliorates acute lung injury induced by blast injury through the DDAH1/ADMA pathwayPubmed:29415054
  • A Novel scFv Anti-Aβ Antibody Reduces Pathological Impairments in APP/PS1 Transgenic Mice via Modulation of Inflammatory Cytokines and Aβ-related EnzymesPubmed:30062438
  • Shock waves increase pulmonary vascular leakage, inflammation, oxidative stress, and apoptosis in a mouse modelPubmed:29984607
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  • Lipoxin A attenuates LPS-induced acute lung injury via activation of the ACE2-Ang-(1-7)-Mas axisPubmed:29969931
  • Genetic and immunogenicity analysis of porcine circovirus type 2 strains isolated in central ChinaPubmed:29305646
  • Effect of tropical thermal stress on peri-implantation immune responses in cowsPubmed:29625402
  • Bushenkangshuai Tablet Reduces Atherosclerotic Lesion by Improving Blood Lipids Metabolism and Inhibiting Inflammatory Response via TLR4 and NF-κB Signaling …Pubmed:29619063
  • Tualang Honey Reduced Neuroinflammation and Caspase-3 Activity in Rat Brain after Kainic Acid-Induced Status EpilepticusPubmed:30108663
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  • S‑adenosyl methionine regulates calcium channels and inhibits uterine smooth muscle contraction in rats with infectious premature delivery through the transient …Pubmed:29896230
  • Comparison of Plasma Interleukin-6 and Interleukin-10 Concentrations between Female Dogs with Mammary Gland Tumours and Healthy17-377.pdf
  • Changes in Th1/Th2-producing cytokines during acute exacerbation chronic obstructive pulmonary diseasePubmed:29950127
  • Effect of reproductive disorders on productivity and reproductive efficiency of dromedary she-camels in relation to cytokine concentrationPubmed:29423707
  • Gastroprotective effect of apricot kernel oil in ethanol-induced gastric mucosal injury in ratsPubmed: 30234391
  • Natural killer T cell ligand alpha-galactosylceramide protects against gut ischemia reperfusion-induced organ injury in micePubmed: 30195979
  • Malaysian propolis, metformin and their combination, exert hepatoprotective effect in streptozotocin-induced diabetic ratsPubmed: 30205096
  • The relationship between autophagy and apoptosis of lamina propria dendritic cells from the small intestine on the immunodynamics of sepsis mice
  • Inhibition of Rho kinase by GSK 269962 reverses both corticosterone-induced detrusor overactivity and depression-like behaviour in ratsPubmed: 30172788
  • PD-1 blockade inhibits lymphocyte apoptosis and restores proliferation and anti-viral immune functions of lymphocyte after CP and NCP BVDV infection in vitroDoi: 10.1016/j.vetmic.2018.10.014
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