CLIA Kit for Lactoferrin (LTF) Bos taurus; Bovine (Cattle) Sandwich CLIA

HLF2; LF; GIG12; Lactotransferrin; Growth-inhibiting protein 12; Talalactoferrin; Lfcin-H; Kaliocin-1

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  • CLIA Kit for Lactoferrin (LTF) Packages (Simulation)
  • CLIA Kit for Lactoferrin (LTF) Packages (Simulation)
  • CLIA Kit for Lactoferrin (LTF) Results demonstration
  • SCA780Bo.jpg Typical Standard Curve
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Recovery

Matrices listed below were spiked with certain level of recombinant Lactoferrin (LTF) and the recovery rates were calculated by comparing the measured value to the expected amount of Lactoferrin (LTF) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 91-99 96
EDTA plasma(n=5) 80-93 85
heparin plasma(n=5) 84-92 88

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Lactoferrin (LTF) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Lactoferrin (LTF) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Lactoferrin (LTF) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 79-92% 78-101% 78-92% 84-101%
EDTA plasma(n=5) 82-97% 90-102% 78-105% 78-89%
heparin plasma(n=5) 83-91% 80-102% 95-103% 90-104%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
Substrate A 1×10mL Substrate B 1×2mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 100µL Substrate Solution. Incubate 10 minutes at 37°C;
8. Read RLU value immediately.

CLIA Kit for Lactoferrin (LTF)

Test principle

The microplate provided in this kit has been pre-coated with an antibody specific to Lactoferrin (LTF). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Lactoferrin (LTF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Lactoferrin (LTF) level in the sample or standard.;

Citations

  • Changes of hepatic lactoferrin gene expression in two mouse models of the acute phase reactionScienceDirect: S1357272511002524
  • Changes in composition of colostrum of Egyptian buffaloes and Holstein cowsBioMed: 17466148
  • p38MAPK, ERK and PI3K Signaling Pathways Are Involved in C5a-Primed Neutrophils for ANCA-Mediated Activation PlosOne: source
  • Sphingolipid Pathway Regulates Innate Immune Responses at the Fetomaternal Interface during PregnancyPubmed:25505239
  • Control of Methicillin-Resistant Staphylococcus aureus Pneumonia Utilizing TLR2 Agonist Pam3CSK4Pubmed:26974438
  • Salivary lactoferrin is transferred into the brain via the sublingual routepubmed:28351211
  • Effects of fluticasone propionate and budesonide on the expression of immune defense genes in bronchial epithelial cellsPubmed:29627483
  • TLR2 agonist Pam3CSK4 enhances the antibacterial functions of GM-CSF induced neutrophils to methicillin-resistant Staphylococcus aureus
  • Assessment of the Diagnostic Value of IL-10 and Lactoferrin by BIRADS Categories in Breast Neoplasms
  • A Three-Protein Panel to Support the Diagnosis of Sepsis in ChildrenPubmed:35329889
  • Three Urban of China: A Cohort Study of Maternal-Infant Factors and HM Protein ComponentsPubmed:35438093
  • Myeloid-Specific Pyruvate-Kinase-Type-M2-Deficient Mice Are Resistant to Acute Lung InjuryPubmed:35625931

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