CLIA Kit for Chemokine (C-X-C Motif) Ligand 1 (CXCL1) Rattus norvegicus (Rat) Sandwich CLIA

NAP3; GRO1; GROa; GRO-A; MGSA-A; NAP3; SCYB1; Chemokine C-X-C-Motif Ligand 1; Melanoma Growth Stimulating Activity Alpha; Fibroblast Secretory Protein

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  • CLIA Kit for Chemokine (C-X-C Motif) Ligand 1 (CXCL1) Packages (Simulation)
  • CLIA Kit for Chemokine (C-X-C Motif) Ligand 1 (CXCL1) Packages (Simulation)
  • CLIA Kit for Chemokine (C-X-C Motif) Ligand 1 (CXCL1) Results demonstration
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Recovery

Matrices listed below were spiked with certain level of recombinant Chemokine (C-X-C Motif) Ligand 1 (CXCL1) and the recovery rates were calculated by comparing the measured value to the expected amount of Chemokine (C-X-C Motif) Ligand 1 (CXCL1) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 80-101 90
EDTA plasma(n=5) 79-101 84
heparin plasma(n=5) 78-89 83

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Chemokine (C-X-C Motif) Ligand 1 (CXCL1) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Chemokine (C-X-C Motif) Ligand 1 (CXCL1) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Chemokine (C-X-C Motif) Ligand 1 (CXCL1) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 83-91% 92-99% 96-104% 78-98%
EDTA plasma(n=5) 97-105% 98-105% 97-105% 97-104%
heparin plasma(n=5) 78-96% 95-102% 87-94% 87-97%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
Substrate A 1×10mL Substrate B 1×2mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 100µL Substrate Solution. Incubate 10 minutes at 37°C;
8. Read RLU value immediately.

CLIA Kit for Chemokine (C-X-C Motif) Ligand 1 (CXCL1)

Test principle

The microplate provided in this kit has been pre-coated with an antibody specific to Chemokine (C-X-C Motif) Ligand 1 (CXCL1). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Chemokine (C-X-C Motif) Ligand 1 (CXCL1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Chemokine (C-X-C Motif) Ligand 1 (CXCL1) level in the sample or standard.;

Citations

  • 白细胞介素10 上调表达对家兔慢性细菌性鼻窦炎黏膜创伤修复的影响article:11593
  • Epidermal CD147 expression plays a key role in IL-22-induced psoriatic dermatitis.pubmed:28272440
  • Total flavonoids from sea buckthorn ameliorates lipopolysaccharide/cigarette smoke‐induced airway inflammationPubmed: 31209984
  • CXCL1 as an Unfavorable Prognosis Factor Negatively Regulated by DACH1 in Non-small Cell Lung CancerPubmed: 31998654
  • Baohuoside I suppresses breast cancer metastasis by downregulating the tumor-associated macrophages/CXC motif chemokine ligand 1 pathwayPubmed: 32911383
  • Extracellular cold-inducible RNA-binding protein regulates neutrophil extracellular trap formation and tissue damage in acute pancreatitisPubmed: 32709888
  • Aiduqing formula suppresses breast cancer metastasis via inhibiting CXCL1-mediated autophagy
  • Aiduqing formula inhibits breast cancer metastasis by suppressing TAM/CXCL1-induced Treg differentiation and infiltration34461944
  • Interleukin-10-Mediated Lymphopenia Caused by Acute Infection with Foot-and-Mouth Disease Virus in Mice34960627

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