Monoclonal Antibody to Cluster Of Differentiation 73 (CD73) Homo sapiens (Human) Monoclonal antibody

NT5E; NT5-E; E5NT; E5-NT; NTE; EN; ENT; 5'-Nucleotidase, Ecto

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Overview
Properties
  • SourceMonoclonal antibody preparation, Host Mouse
  • Ig Isotype IgG2b Kappa, Clone Number D1
  • PurificationProtein A + Protein G affinity chromatography
  • LabelNone
  • Immunogen RPB250Hu01-Recombinant Cluster Of Differentiation 73 (CD73)
  • Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
  • TraitsLiquid, Concentration 1mg/mL
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  • Monoclonal Antibody to Cluster Of Differentiation 73 (CD73) Packages (Simulation)
  • Monoclonal Antibody to Cluster Of Differentiation 73 (CD73) Packages (Simulation)
  • Monoclonal Antibody to Cluster Of Differentiation 73 (CD73) DAB staining on IHC-P; Sample: Human Placenta Tissue; Primary Ab: 10µg/ml Mouse Anti-Human CD73 Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
  • Monoclonal Antibody to Cluster Of Differentiation 73 (CD73) DAB staining on IHC-P;
    Sample: Human Small intestine Tissue;
    Primary Ab: 10µg/ml Mouse Anti-Human CD73 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu19)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a mouse monoclonal antibody raised against CD73. It has been selected for its ability to recognize CD73 in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.5-2µg/mL
Immunohistochemistry: 5-20µg/mL
Immunocytochemistry: 5-20µg/mL
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Organism Species More: Sus scrofa; Porcine (Pig)

Giveaways

Citations

  • The expression of CD39 on regulatory T cells is genetically driven and further upregulated at sites of inflammationPubmed:25640206
  • Simultaneous accurate quantification of HO-1, CD39, and CD73 in human calcified aortic valves using multiple enzyme digestion–filter aided sample pretreatment …10.1016:j.talanta.2018.01.044
  • Expression and clinical significance of serum NT5E protein in patients with colorectal cancerPubmed: 30932882

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