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ITEM PROTEIN ANTIBODY ASSAY KIT CELL
Polyclonal Antibody to Aldehyde Dehydrogenase 7 Family, Member A1 (ALDH7A1)
ATQ1; PDE; EPD; Antiquitin; Alpha-Aminoadipic Semialdehyde Dehydrogenase; Delta1-Piperideine-6-Carboxylate Dehydrogenease; P6c Dehydrogenase
Organism species Homo sapiens (Human)
Product No.PAE837Hu01
ClonalityPolyclonal
HostRabbit
Immunoglobulin TypeIgG
PurificationAffinity Chromatography
ApplicationsWB, ICC, IHC-P, IHC-F, ELISA
Concentration200ug/ml
UOM100ug
ConjugateNo Conjugate
IMMUNOGEN INFORMATION
Immunogen: Recombinant ALDH7A1 (Met1~Gln539) expressed in E.coli.
Accession No.: RPE837Hu01
Sequence: The target protein is fused with a His-tag and its sequence is listed below.
MGHHHHHHSGS-MWRLPRALCV HAAKTSKLSG PWSRPAAFMS TLLINQPQYA WLKELGLREE NEGVYNGSWG GRGEVITTYC PANNEPIARV RQASVADYEE TVKKAREAWK IWADIPAPKR GEIVRQIGDA LREKIQVLGS LVSLEMGKIL VEGVGEVQEY VDICDYAVGL SRMIGGPILP SERSGHALIE QWNPVGLVGI ITAFNFPVAV YGWNNAIAMI CGNVCLWKGA PTTSLISVAV TKIIAKVLED NKLPGAICSL TCGGADIGTA MAKDERVNLL SFTGSTQVGK QVGLMVQERF GRSLLELGGN NAIIAFEDAD LSLVVPSALF AAVGTAGQRC TTARRLFIHE SIHDEVVNRL KKAYAQIRVG NPWDPNVLYG PLHTKQAVSM FLGAVEEAKK EGGTVVYGGK VMDRPGNYVE PTIVTGLGHD ASIAHTETFA PILYVFKFKN EEEVFAWNNE VKQGLSSSIF TKDLGRIFRW LGPKGSDCGI VNVNIPTSGA EIGGAFGGEK HTGGGRESGS DAWKQYMRRS TCTINYSKDL PLAQGIKFQ
ANTIBODY SPECIFITY
The antibody is a rabbit polyclonal antibody raised against ALDH7A1. It has been selected for its ability to recognize ALDH7A1 in immunohistochemical staining and western blotting.
APPLICATIONS
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.
CONTENTS
Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
QUALITY CONTROL
Content: The quality control contains recombinant Thyroglobulin (Ser2083~Ala2333) disposed in loading buffer.
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
                5uL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control. Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol, BPB 0.01%, NaN3 0.02%.
STORAGE
Store at 4oC for frequent use. Stored at -20oC to -80oC in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Package and Components
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Benchmark price
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications
PAE837Hu01Polyclonal Antibody to Aldehyde Dehydrogenase 7 Family, Member A1 (ALDH7A1)WB, ICC, IHC-P, IHC-F, ELISA
PAE837Hu71Biotin-Linked Antibody to Aldehyde Dehydrogenase 7 Family, Member A1 (ALDH7A1)IHC;WB;ELISA
RPE837Hu01Recombinant Aldehyde Dehydrogenase 7 Family, Member A1 (ALDH7A1)SDS-PAGE; WB; ELISA; IP.