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ITEM PROTEIN ANTIBODY ASSAY KIT CELL
Polyclonal Antibody to Amyloid Precursor Protein (APP)
AAA; ABeta; ABPP; AD1; APPI; CVAP; PN2; CTFgamma; Peptidase Nexin-II Alzheimer Disease; Peptidase Nexin-II; Amyloid Beta(A4)Precursor Protein
Organism species Homo sapiens (Human)
Product No.PAB020Hu01
ClonalityPolyclonal
HostRabbit
Immunoglobulin TypeIgG
PurificationAffinity Chromatography
ApplicationsWB, ICC, IHC-P, IHC-F, ELISA
Concentration200ug/ml
UOM100ug
ConjugateNo Conjugate
IMMUNOGEN INFORMATION
Immunogen: Recombinant APP (Asp672~Val711) expressed in E.coli.
Accession No.: RPB020Hu01
Sequence: The target protein is fused with two N-terminal Tags, His-tag and MBP-tag and its sequence is listed below.
MKIEEGKLVI WINGDKGYNG LAEVGKKFEK DTGIKVTVEH PDKLEEKFPQ VAATGDGPDI IFWAHDRFGG YAQSGLLAEI TPDKAFQDKL YPFTWDAVRY NGKLIAYPIA VEALSLIYNK DLLPNPPKTW EEIPALDKEL KAKGKSALMF NLQEPYFTWP LIAADGGYAF KYENGKYDIK DVGVDNAGAK AGLTFLVDLI KNKHMNADTD YSIAEAAFNK GETAMTINGP WAWSNIDTSK VNYGVTVLPT FKGQPSKPFV GVLSAGINAA SPNKELAKEF LENYLLTDEG LEAVNKDKPL GAVALKSYEE ELAKDPRIAA TMENAQKGEI MPNIPQMSAF WYAVRTAVIN AASGRQTVDE ALKDAQTGST SGSGHHHHHH SAGLVPRGST AIGMKETAAA KFERQHMDSP DLGTLEVLFQ GPLGS- DAEFRHDSG YEVHHQKLVF FAEDVGSNKG AIIGLMVGGV V
ANTIBODY SPECIFITY
The antibody is a rabbit polyclonal antibody raised against APP. It has been selected for its ability to recognize APP in immunohistochemical staining and western blotting.
APPLICATIONS
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.
CONTENTS
Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
QUALITY CONTROL
Content: The quality control contains recombinant APP (Asp672~Val711) disposed in loading buffer.
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
                5uL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control. Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol, BPB 0.01%, NaN3 0.02%.
STORAGE
Store at 4oC for frequent use. Stored at -20oC to -80oC in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Package and Components
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Benchmark price
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications
MAB020Hu22Monoclonal Antibody to Amyloid Precursor Protein (APP)WB, ICC, IHC-P, IHC-F, ELISA
PAB020Hu01Polyclonal Antibody to Amyloid Precursor Protein (APP)WB, ICC, IHC-P, IHC-F, ELISA
RPB020Hu01Recombinant Amyloid Precursor Protein (APP)SDS-PAGE; WB; ELISA; IP.
SCB020HuCLIA Kit for Amyloid Precursor Protein (APP)Chemiluminescent immunoassay
SEB020HuELISA Kit for Amyloid Precursor Protein (APP)Enzyme-linked immunosorbent assay