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ITEM PROTEIN ANTIBODY ASSAY KIT CELL
Polyclonal Antibody to Calmodulin 1 (CALM1)
CALML2; CAMI; DD132; PHKD; Phosphorylase Kinase,Delta
Organism species Homo sapiens (Human)
Product No.PAD315Hu01
ClonalityPolyclonal
HostRabbit
Immunoglobulin TypeIgG
PurificationAffinity Chromatography
ApplicationsWB, ICC, IHC-P, IHC-F, ELISA
Concentration200ug/ml
UOM100ug
ConjugateNo Conjugate
IMMUNOGEN INFORMATION
Immunogen: Recombinant CALM1 (Met1~Lys149) expressed in E.coli.
Accession No.: RPD315Hu01
Sequence: The target protein is fused with two N-terminal Tags, His-tag and GST-tag and its sequence is listed below.
MRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTLEV LFGPLGSEF-MADQLTEEQI AEFKEAFSLF DKDGDGTITT KELGTVMRSL GQNPTEAELQ DMINEVDADG NGTIDFPEFL TMMARKMKDT DSEEEIREAF RVFDKDGNGY ISAAELRHVM TNLGEKLTDE EVDEMIREAD IDGDGQVNYE EFVQMMTAK
ANTIBODY SPECIFITY
The antibody is a rabbit polyclonal antibody raised against CALM1. It has been selected for its ability to recognize CALM1 in immunohistochemical staining and western blotting.
APPLICATIONS
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.
CONTENTS
Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
QUALITY CONTROL
Content: The quality control contains recombinant CALM1 (Met1~Lys149) disposed in loading buffer.
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
                5uL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control. Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol, BPB 0.01%, NaN3 0.02%.
STORAGE
Store at 4oC for frequent use. Stored at -20oC to -80oC in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Package and Components
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Benchmark price
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications
PAD315Hu01Polyclonal Antibody to Calmodulin 1 (CALM1)WB, ICC, IHC-P, IHC-F, ELISA
PAD315Hu02Polyclonal Antibody to Calmodulin 1 (CALM1)WB, ICC, IHC-P, IHC-F, ELISA
PAD315Hu71Biotin-Linked Antibody to Calmodulin 1 (CALM1)IHC;WB;ELISA
RPD315Hu01Recombinant Calmodulin 1 (CALM1)SDS-PAGE; WB; ELISA; IP.
RPD315Hu02Recombinant Calmodulin 1 (CALM1)SDS-PAGE; WB; ELISA; IP.