Polyclonal Antibody to Carnitine Palmitoyltransferase 2, Mitochondrial (CPT2)

CPTASE; Carnitine O-Palmitoyltransferase 2; Carnitine palmitoyltransferase II
Western Blot

IMMUNOGEN INFORMATIONImmunogen: Recombinant CPT2 (Ala152~Ser658) expressed in E.coli.
Accession No.: RPC417Hu01
Sequence: The target protein is fused with two N-terminal Tags, His-tag and GST-tag and its sequence is listed below.
ANTIBODY SPECIFITY The antibody is a rabbit polyclonal antibody raised against CPT2. It has been selected for its ability to recognize CPT2 in immunohistochemical staining and western blotting. APPLICATIONS WB, ICC, IHC-P, IHC-F, ELISA
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.
CONTENTS Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol. QUALITY CONTROL Content: The quality control contains recombinant CPT2 (Ala152~Ser658) disposed in loading buffer.
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
                5uL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control. Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol, BPB 0.01%, NaN3 0.02%.
STORAGE Store at 4oC for frequent use. Stored at -20oC to -80oC in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

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    Polyclonal Antibody to Carnitine Palmitoyltransferase 2, Mitochondrial (CPT2) Material Safety Data Sheet International Organization for Standardization guarantee