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ITEM PROTEIN ANTIBODY ASSAY KIT CELL
Polyclonal Antibody to Cathepsin A (CTSA)
CTS-A; GLB2; GSL; NGBE; PPCA; PPGB; Protective Protein For Beta-Galactosidase; Galactosialidosis
Organism species Homo sapiens (Human)
Product No.PAA348Hu01
ClonalityPolyclonal
HostRabbit
Immunoglobulin TypeIgG
PurificationAffinity Chromatography
ApplicationsWB, ICC, IHC-P, IHC-F, ELISA
Concentration200ug/ml
UOM100ug
ConjugateNo Conjugate
IMMUNOGEN INFORMATION
Immunogen: Recombinant CTSA (Asp215~Met470) expressed in E.coli.
Accession No.: RPA348Hu01
Sequence: The target protein is fused with two N-terminal Tags, His-tag and GST-tag and its sequence is listed below.
MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQGPLGSEF-DNSLVY FAYYHGLLGN RLWSSLQTHC CSQNKCNFYD NKDLECVTNL QEVARIVGNS GLNIYNLYAP CAGGVPSHFR YEKDTVVVQDLGNIFTRLPL KRMWHQALLR SGDKVRMDPP CTNTTAASTY LNNPYVRKAL NIPEQLPQWD MCNFLVNLQY RRLYRSMNSQ YLKLLSSQKY QILLYNGDVD MACNFMGDEW FVDSLNQKMEVQRRPWLVKY GDSGEQIAGF VKEFSHIAFL TIKGAGHMVP TDKPLAAFTM
ANTIBODY SPECIFITY
The antibody is a rabbit polyclonal antibody raised against CTSA. It has been selected for its ability to recognize CTSA in immunohistochemical staining and western blotting.
APPLICATIONS
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.
CONTENTS
Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
QUALITY CONTROL
Content: The quality control contains recombinant CTSA (Asp215~Met470) disposed in loading buffer.
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
                5uL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control. Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol, BPB 0.01%, NaN3 0.02%.
STORAGE
Store at 4oC for frequent use. Stored at -20oC to -80oC in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Package and Components
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Benchmark price
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications
MAA348Hu21Monoclonal Antibody to Cathepsin A (CTSA)WB, ICC, IHC-P, IHC-F, ELISA
PAA348Hu01Polyclonal Antibody to Cathepsin A (CTSA)WB, ICC, IHC-P, IHC-F, ELISA
PAA348Hu71Biotin-Linked Antibody to Cathepsin A (CTSA)IHC;WB;ELISA
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RPA348Hu01Recombinant Cathepsin A (CTSA)SDS-PAGE; WB; ELISA; IP.
SEA348HuELISA Kit for Cathepsin A (CTSA)Enzyme-linked immunosorbent assay