IMMUNOGEN INFORMATIONImmunogen: Recombinant CYLD (Glu170~Gly338) expressed in E.coli.
Accession No.: RPC423Hu01
Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- E GRGQGFTDGV YQGKQLFQCD EDCGVFVALD KLELIEDDDT ALESDYAGPG DTMQVELPPL EINSRVSLKV GETIESGTVI FCDVLPGKES LGYFVGVDMD NPIGNWDGRF DGVQLCSFAC VESTILLHIN DIIPESVTQE RRPPKLAFMS RGVGDKGSSS HNKPKATG ANTIBODY SPECIFITY The antibody is a rabbit polyclonal antibody raised against CYLD. It has been selected for its ability to recognize CYLD in immunohistochemical staining and western blotting. APPLICATIONS WB, ICC, IHC-P, IHC-F, ELISA
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.
CONTENTS Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol. QUALITY CONTROL Content: The quality control contains recombinant CYLD (Glu170~Gly338) disposed in loading buffer.
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
5uL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control. Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol, BPB 0.01%, NaN3 0.02%. STORAGE Store at 4oC for frequent use. Stored at -20oC to -80oC in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.