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ITEM PROTEIN ANTIBODY ASSAY KIT CELL
Polyclonal Antibody to Epiphycan (EPYC)
DSPG3; Pg-Lb; SLRR3B; Dermatan Sulphate Proteoglycan 3; Dermatan Sulfate Proteoglycan 3
Organism species Homo sapiens (Human)
Product No.PAJ227Hu01
ClonalityPolyclonal
HostRabbit
Immunoglobulin TypeIgG
PurificationAffinity Chromatography
ApplicationsWB, ICC, IHC-P, IHC-F, ELISA
Concentration200ug/ml
UOM100ug
ConjugateNo Conjugate
IMMUNOGEN INFORMATION
Immunogen: Recombinant EPYC (His110~Pro317) expressed in E.coli.
Accession No.: RPJ227Hu01
Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- H TNEDFPTCLL CTCISTTVYC DDHELDAIPP LPKNTAYFYS RFNRIKKINK NDFASLSDLK RIDLTSNLIS EIDEDAFRKL PQLRELVLRD NKIRQLPELP TTLTFIDISN NRLGRKGIKQ EAFKDMYDLH HLYLTDNNLD HIPLPLPENL RALHLQNNNI LEMHEDTFCN VKNLTYIRKA LEDIRLDGNP INLSKTPQAY MCLPRLP
ANTIBODY SPECIFITY
The antibody is a rabbit polyclonal antibody raised against EPYC. It has been selected for its ability to recognize EPYC in immunohistochemical staining and western blotting.
APPLICATIONS
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.
CONTENTS
Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
QUALITY CONTROL
Content: The quality control contains recombinant EPYC (His110~Pro317) disposed in loading buffer.
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
                5uL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control. Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol, BPB 0.01%, NaN3 0.02%.
STORAGE
Store at 4oC for frequent use. Stored at -20oC to -80oC in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Package and Components
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Benchmark price
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications
MAJ227Hu21Monoclonal Antibody to Epiphycan (EPYC)WB, ICC, IHC-P, IHC-F, ELISA
PAJ227Hu01Polyclonal Antibody to Epiphycan (EPYC)WB, ICC, IHC-P, IHC-F, ELISA
RPJ227Hu01Recombinant Epiphycan (EPYC)SDS-PAGE; WB; ELISA; IP.
SEJ227HuELISA Kit for Epiphycan (EPYC)Enzyme-linked immunosorbent assay