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ITEM PROTEIN ANTIBODY ASSAY KIT CELL
Polyclonal Antibody to Fibroblast Growth Factor Receptor 2 (FGFR2)
BEK; CD332; BFR1; CEK3; CFD1; ECT1; JWS; K-SAM; KGFR; TK14; Bacteria-Expressed Kinase,Keratinocyte Growth Factor Receptor; Craniofacial Dysostosis 1; Crouzon Syndrome
Organism species Homo sapiens (Human)
Product No.PAB794Hu01
ClonalityPolyclonal
HostRabbit
Immunoglobulin TypeIgG
PurificationAffinity Chromatography
ApplicationsWB, ICC, IHC-P, IHC-F, ELISA
Concentration200ug/ml
UOM100ug
ConjugateNo Conjugate
IMMUNOGEN INFORMATION
Immunogen: Recombinant FGFR2 (Thr157~Glu377) expressed in E.coli.
Accession No.: RPB794Hu01
Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- TNTE KMEKRLHAVP AANTVKFRCP AGGNPMPTMR WLKNGKEFKQ EHRIGGYKVR NQHWSLIMES VVPSDKGNYT CVVENEYGSI NHTYHLDVVE RSPHRPILQA GLPANASTVV GGDVEFVCKV YSDAQPHIQW IKHVEKNGSK YGPDGLPYLK VLKAAGVNTT DKEIEVLYIR NVTFEDAGEY TCLAGNSIGI SFHSAWLTVL PAPGREKEIT ASPDYLE
ANTIBODY SPECIFITY
The antibody is a rabbit polyclonal antibody raised against FGFR2. It has been selected for its ability to recognize FGFR2 in immunohistochemical staining and western blotting.
APPLICATIONS
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.
CONTENTS
Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
QUALITY CONTROL
Content: The quality control contains recombinant Thyroglobulin (Ser2083~Ala2333) disposed in loading buffer.
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
                5uL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control. Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol, BPB 0.01%, NaN3 0.02%.
STORAGE
Store at 4oC for frequent use. Stored at -20oC to -80oC in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Package and Components
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Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications
MAB794Hu22Monoclonal Antibody to Fibroblast Growth Factor Receptor 2 (FGFR2)WB, ICC, IHC-P, IHC-F, ELISA
PAB794Hu01Polyclonal Antibody to Fibroblast Growth Factor Receptor 2 (FGFR2)WB, ICC, IHC-P, IHC-F, ELISA
RPB794Hu01Recombinant Fibroblast Growth Factor Receptor 2 (FGFR2)SDS-PAGE; WB; ELISA; IP.
SEB794HuELISA Kit for Fibroblast Growth Factor Receptor 2 (FGFR2)Enzyme-linked immunosorbent assay