Home  |   About us
ITEM PROTEIN ANTIBODY ASSAY KIT CELL
Polyclonal Antibody to Fibronectin Type III Domain Containing Protein 5 (FNDC5)
FRCP2; Irisin
Organism species Homo sapiens (Human)
Product No.PAN576Hu01
ClonalityPolyclonal
HostRabbit
Immunoglobulin TypeIgG
PurificationAffinity Chromatography
ApplicationsWB, ICC, IHC-P, IHC-F, ELISA
Concentration200ug/ml
UOM100ug
ConjugateNo Conjugate
IMMUNOGEN INFORMATION
Immunogen: Recombinant FNDC5 (Leu2~Ile137) expressed in E.coli.
Accession No.: RPN576Hu01
Sequence: The target protein is fused with N-terminal His-Tag and its sequence is listed below.
MGHHHHHHSGSEF- LRFIQEVNT TTRSCALWDL EEDTEYIVHV QAISIQGQSP ASEPVLFKTP REAEKMASKN KDEVTMKEMG RNQQLRTGEV LIIVVVLFMW AGVIALFCRQ YDIIKDNEPN NNKEKTKSAS ETSTPEHQGG GLLRSKI
ANTIBODY SPECIFITY
The antibody is a rabbit polyclonal antibody raised against FNDC5. It has been selected for its ability to recognize FNDC5 in immunohistochemical staining and western blotting.
APPLICATIONS
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.
CONTENTS
Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
QUALITY CONTROL
Content: The quality control contains recombinant FNDC5 (Leu2~Ile137) disposed in loading buffer.
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
                5uL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control. Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol, BPB 0.01%, NaN3 0.02%.
STORAGE
Store at 4oC for frequent use. Stored at -20oC to -80oC in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Package and Components
Download
Benchmark price
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications
PAN576Hu01Polyclonal Antibody to Fibronectin Type III Domain Containing Protein 5 (FNDC5)WB, ICC, IHC-P, IHC-F, ELISA
PAN576Hu02Polyclonal Antibody to Fibronectin Type III Domain Containing Protein 5 (FNDC5)WB, ICC, IHC-P, IHC-F, ELISA
PAN576Hu71Biotin-Linked Antibody to Fibronectin Type III Domain Containing Protein 5 (FNDC5)IHC;WB;ELISA
RPN576Hu01Recombinant Fibronectin Type III Domain Containing Protein 5 (FNDC5)SDS-PAGE; WB; ELISA; IP.
RPN576Hu02Recombinant Fibronectin Type III Domain Containing Protein 5 (FNDC5)SDS-PAGE; WB; ELISA; IP.
SEN576HuELISA Kit for Fibronectin Type III Domain Containing Protein 5 (FNDC5)Enzyme-linked immunosorbent assay