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ITEM PROTEIN ANTIBODY ASSAY KIT CELL
Polyclonal Antibody to Hexokinase 2 (HK2)
Organism species Homo sapiens (Human)
Product No.PAD352Hu01
ClonalityPolyclonal
HostRabbit
Immunoglobulin TypeIgG
PurificationAffinity Chromatography
ApplicationsWB, ICC, IHC-P, IHC-F, ELISA
Concentration200ug/ml
UOM100ug
ConjugateNo Conjugate
IMMUNOGEN INFORMATION
Immunogen: Recombinant HK2 (Trp619~Arg917) expressed in E.coli.
Accession No.: RPD352Hu01
Sequence: The target protein is fused with N-terminal His-Tag and its sequence is listed below.
MGHHHHHHSG SEF-WT KGFKASGCEG EDVVTLLKEA IHRREEFDLD VVAVVNDTVG TMMTCGFEDP HCEVGLIVGT GSNACYMEEM RNVELVEGEE GRMCVNMEWG AFGDNGCLDD FRTEFDVAVD ELSLNPGKQR FEKMISGMYL GEIVRNILID FTKRGLLFRG RISERLKTRG IFETKFLSQI ESDCLALLQV RAILQHLGLE STCDDSIIVK EVCTVVARRA AQLCGAGMAA VVDRIRENRG LDALKVTVGV DGTLYKLHPH FAKVMHETVK DLAPKCDVSF LQSEDGSGKG AALITAVACR IREAGQR
ANTIBODY SPECIFITY
The antibody is a rabbit polyclonal antibody raised against HK2. It has been selected for its ability to recognize HK2 in immunohistochemical staining and western blotting.
APPLICATIONS
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.
CONTENTS
Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
QUALITY CONTROL
Content: The quality control contains recombinant HK2 (Trp619~Arg917) disposed in loading buffer.
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
                5uL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control. Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol, BPB 0.01%, NaN3 0.02%.
STORAGE
Store at 4oC for frequent use. Stored at -20oC to -80oC in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Package and Components
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Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications
MAD352Hu21Monoclonal Antibody to Hexokinase 2 (HK2)WB, ICC, IHC-P, IHC-F, ELISA
PAD352Hu01Polyclonal Antibody to Hexokinase 2 (HK2)WB, ICC, IHC-P, IHC-F, ELISA
PAD352Hu02Polyclonal Antibody to Hexokinase 2 (HK2)WB, ICC, IHC-P, IHC-F, ELISA
RPD352Hu01Recombinant Hexokinase 2 (HK2)SDS-PAGE; WB; ELISA; IP.
RPD352Hu02Recombinant Hexokinase 2 (HK2)SDS-PAGE; WB; ELISA; IP.
SED352HuELISA Kit for Hexokinase 2 (HK2)Enzyme-linked immunosorbent assay