Polyclonal Antibody to Erythropoietin (EPO)Homo sapiens (Human)Polyclonal antibody

EP; Epoetin; Erythropoetin; Hematopoietin; Hemopoietin

  • Product No.PAA028Hu01
  • Organism SpeciesHomo sapiens (Human)Same name, Different species.
  • SourcePolyclonal antibody preparation
  • HostRabbit
  • Potencyn/a
  • Ig TypeIgG
  • PurificationAntigen-specific affinity chromatography
  • LabelNone
  • Immunogenn/a
  • Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
  • TraitsLiquid
  • Concentration200ug/ml
  • Organism Species Moren/a
  • ApplicationsWB, ICC, IHC-P, IHC-F, ELISA
  • DownloadInstruction Manual
  • UOM10ug50ug100ug1mg1g
  • FOBUS$ 84US$ 196US$ 280US$ 1120US$ 84000
  • Polyclonal Antibody to Erythropoietin (EPO)Package and Components
  • Polyclonal Antibody to Erythropoietin (EPO)Package and Components
  • Polyclonal Antibody to Erythropoietin (EPO)Used in DAB staining on fromalin fixed paraffin-embedded Liver tissue
  • PAA028Hu01.jpgWB Image
  • CertificateISO9001: 2008, ISO13485: 2003 Registered

SPECIFITY

The antibody is a rabbit polyclonal antibody raised against EPO. It has been selected for its ability to recognize EPO in immunohistochemical staining and western blotting.

USAGE

Western blotting: 1:50-400
Immunocytochemistry in formalin fixed cells: 1:50-500
Immunohistochemistry in formalin fixed frozen section: 1:50-500
Immunohistochemistry in paraffin section: 1:10-100
Enzyme-linked Immunosorbent Assay: 1:100-1:5000
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Reference

  • EndocrinologyPrenatal Glucocorticoid Overexposure Causes Permanent Increases in Renal Erythropoietin Expression and Red Blood Cell Mass in the Rat OffspringEndo: source
  • Journal of EthnopharmacologyInhibitory effect of polysaccharides isolated from Angelica sinensis on hepcidin expressionPubMed: 21333724
  • PLOS ONECD98 Positive Eosinophils Contribute to T Helper 1 Pattern InflammationPlosOne: Source
  • The Journal of Clinical Endocrinology & MetabolismAlterations of Circulating Endothelial Cell and Endothelial Progenitor Cell Counts around the OvulationPubMed: 22948762
  •  Experimental & Molecular MedicineLong-term and stable correction of uremic anemia by intramuscular injection of plasmids containing hypoxia-regulated system of erythropoietin expressionPubMed: PMC3509184
  • Biochem Biophys Res Commun.Reevaluation of erythropoietin production by the nephronPubmed:24832733
  • Nutrition. Carbohydrate and glutamine supplementation modulates the Th1/Th2 balance after exercise performed at a simulated altitude of 4500 m.Pubmed:2528040
  • J Appl Physiol (1985).Decreased plasma soluble erythropoietin receptor in high-altitude excessive erythrocytosis and Chronic Mountain SicknessPubmed:25324511
  • Expert Rev ProteomicsPlasma hepcidin in early-stage breast cancer patients: no relationship with interleukin-6, erythropoietin and erythroferronePubMed: 26496240