IMMUNOGEN INFORMATIONImmunogen: Recombinant Native Protein INS expressed in E.coli.
Accession No.: RPA448Hu01
Insulin is a peptide hormone, produced by beta cells of the pancreas, and is central to regulating carbohydrate and fat metabolism in the body. Insulin causes cells in the liver, skeletal muscles, and fat tissue to absorb glucose from the blood. In the liver and skeletal muscles, glucose is stored as glycogen, and in fat cells it is stored as triglycerides. The human insulin protein is composed of 51 amino acids, and has a molecular weight of 5808Da. It is a dimer of an A-chain and a B-chain, which are linked together by disulfide bonds. ANTIBODY SPECIFITY The antibody is a rabbit polyclonal antibody raised against INS. It has been selected for its ability to recognize INS in immunohistochemical staining and western blotting. APPLICATIONS WB, ICC, IHC-P, IHC-F, ELISA
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.
CONTENTS Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol. STORAGE Store at 4oC for frequent use. Stored at -20oC to -80oC in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.