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ITEM PROTEIN ANTIBODY ASSAY KIT CELL
Polyclonal Antibody to Insulin (INS)
Organism species Homo sapiens (Human)
Product No.PAA448Hu01
ClonalityPolyclonal
HostRabbit
Immunoglobulin TypeIgG
PurificationAffinity Chromatography
ApplicationsWB, ICC, IHC-P, IHC-F, ELISA
Concentration200ug/ml
UOM100ug
ConjugateNo Conjugate
IMMUNOGEN INFORMATION
Immunogen: Recombinant Native Protein INS expressed in E.coli.
Accession No.: RPA448Hu01
Insulin is a peptide hormone, produced by beta cells of the pancreas, and is central to regulating carbohydrate and fat metabolism in the body. Insulin causes cells in the liver, skeletal muscles, and fat tissue to absorb glucose from the blood. In the liver and skeletal muscles, glucose is stored as glycogen, and in fat cells it is stored as triglycerides. The human insulin protein is composed of 51 amino acids, and has a molecular weight of 5808Da. It is a dimer of an A-chain and a B-chain, which are linked together by disulfide bonds.
ANTIBODY SPECIFITY
The antibody is a rabbit polyclonal antibody raised against INS. It has been selected for its ability to recognize INS in immunohistochemical staining and western blotting.
APPLICATIONS
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.
CONTENTS
Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
STORAGE
Store at 4oC for frequent use. Stored at -20oC to -80oC in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Package and Components
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Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications
CEA448HuELISA Kit for Insulin (INS)Enzyme-linked immunosorbent assay
MAA448Hu22Monoclonal Antibody to Insulin (INS)WB, ICC, IHC-P, IHC-F, ELISA
NPA448Hu01Native Insulin (INS)SDS-PAGE; WB; ELISA; IP.
PAA448Hu01Polyclonal Antibody to Insulin (INS)WB, ICC, IHC-P, IHC-F, ELISA
PAA448Hu71Biotin-Linked Antibody to Insulin (INS)IHC;WB;ELISA
PAA448Hu81FITC-Linked Antibody to Insulin (INS)IHC;ICC;IF;WB
RPA448Hu01Recombinant Insulin (INS)SDS-PAGE; WB; ELISA; IP.
SCA448HuCLIA Kit for Insulin (INS)Chemiluminescent immunoassay
SEA448HuELISA Kit for Insulin (INS)Enzyme-linked immunosorbent assay