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ITEM PROTEIN ANTIBODY ASSAY KIT CELL
Polyclonal Antibody to Leptin (LEP)
Obesity Homolog
Organism species Homo sapiens (Human)
Product No.PAA084Hu01
ClonalityPolyclonal
HostRabbit
Immunoglobulin TypeIgG
PurificationAffinity Chromatography
ApplicationsWB, ICC, IHC-P, IHC-F, ELISA
Concentration200ug/ml
UOM100ug
ConjugateNo Conjugate
IMMUNOGEN INFORMATION
Immunogen: Recombinant LEP (Val22~Cys167) expressed in E.coli.
Accession No.: RPA084Hu01
Sequence: The target protein is fused with a His-tag and its sequence is listed below.
MGHHHHHHSGS-VPIQKVQDD TKTLIKTIVT RINDISHTQS VSSKQKVTGL DFIPGLHPIL TLSKMDQTLA VYQQILTSMP SRNVIQISND LENLRDLLHV LAFSKSCHLP WASGLETLDS LGGVLEASGY STEVVALSRL QGSLQDMLWQ LDLSPGC
ANTIBODY SPECIFITY
The antibody is a rabbit polyclonal antibody raised against LEP. It has been selected for its ability to recognize LEP in immunohistochemical staining and western blotting.
APPLICATIONS
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.
CONTENTS
Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
QUALITY CONTROL
Content: The quality control contains recombinant LEP (Val22~Cys167) disposed in loading buffer.
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
                5uL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control. Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol, BPB 0.01%, NaN3 0.02%.
STORAGE
Store at 4oC for frequent use. Stored at -20oC to -80oC in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Package and Components
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Benchmark price
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications
MAA084Hu22Monoclonal Antibody to Leptin (LEP)WB, ICC, IHC-P, IHC-F, ELISA
PAA084Hu01Polyclonal Antibody to Leptin (LEP)WB, ICC, IHC-P, IHC-F, ELISA
PAA084Hu71Biotin-Linked Antibody to Leptin (LEP)IHC;WB;ELISA
PAA084Hu81FITC-Linked Antibody to Leptin (LEP)IHC;ICC;IF;WB
RPA084Hu01Recombinant Leptin (LEP)SDS-PAGE; WB; ELISA; IP.
SCA084HuCLIA Kit for Leptin (LEP)Chemiluminescent immunoassay
SEA084HuELISA Kit for Leptin (LEP)Enzyme-linked immunosorbent assay