• KO-Validated
Polyclonal Antibody to Tumor Protein p53 (TP53) Homo sapiens (Human) Polyclonal antibody

p53; LFS1; TRP53; Li-Fraumeni Syndrome; Cellular tumor antigen p53; Antigen NY-CO-13; Phosphoprotein p53; Tumor suppressor p53

  • Polyclonal Antibody to Tumor Protein p53 (TP53) Packages (Simulation)
  • Polyclonal Antibody to Tumor Protein p53 (TP53) Packages (Simulation)
  • Polyclonal Antibody to Tumor Protein p53 (TP53) Used in DAB staining on fromalin fixed paraffin-embedded Kidney tissue
  • PAA928Hu01.jpg Figure 1. Western Blot; Sample: Recombinant TP53, Human.
  • Polyclonal Antibody to Tumor Protein p53 (TP53) Western Blot; Sample: Human MCF7 cell lysate;
    Primary Ab: 1µg/ml Rabbit Anti-Human TP53 Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Tumor Protein p53 (TP53) Knockout Varification:
    Lane 1: Wild-type MCF7 cell lysate;
    Lane 2: TP53 knockout MCF7 cell lysate;
    Predicted MW: 24,30,33,34,38,39,44kDa
    Observed MW: 50kDa
    Primary Ab: 1µg/ml Rabbit Anti-Human TP53 Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

SPECIFITY

The antibody is a rabbit polyclonal antibody raised against TP53. It has been selected for its ability to recognize TP53 in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.5-2μg/mL
Immunohistochemistry: 5-20μg/mL
Immunocytochemistry: 5-20μg/mL
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Reference

  • Anticancer ResearchAnalysis of p53 and miRNA expression after irradiation of glioblastoma cell linesPubMed: 23155233
  • Microscopy Research and TechniqueTherapeutic role of curcumin in oxidative DNA damage caused by formaldehydePubMed: 25761397
  • J Nutr BiochemSupplementation with Selenium yeast on the prooxidant–antioxidant activities and anti-tumor effects in breast tumor xenograft-bearing micePubMed: 26344777

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