Polyclonal Antibody to Tumor Protein p53 (TP53)Homo sapiens (Human)Polyclonal antibody

p53; LFS1; TRP53; Li-Fraumeni Syndrome; Cellular tumor antigen p53; Antigen NY-CO-13; Phosphoprotein p53; Tumor suppressor p53

  • Product No.PAA928Hu01
  • Organism SpeciesHomo sapiens (Human)
  • SourcePolyclonal antibody preparation
  • HostRabbit
  • Potencyn/a
  • Ig TypeIgG
  • PurificationAntigen-specific affinity chromatography
  • LabelNone
  • ImmunogenRPA928Hu01-Recombinant Tumor Protein p53 (TP53)
  • Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
  • TraitsLiquid
  • Concentration200ug/ml
  • Organism Species Moren/a
  • ApplicationsWB; ICC; IHC-P; IHC-F; ELISA; IP; IF; FCM.
  • DownloadInstruction Manual
  • UOM10ug50ug100ug1mg1g
  • FOBUS$ 95US$ 221US$ 316US$ 1264US$ 94800
  • Polyclonal Antibody to Tumor Protein p53 (TP53)Package and Components
  • Polyclonal Antibody to Tumor Protein p53 (TP53)Package and Components
  • Polyclonal Antibody to Tumor Protein p53 (TP53)Used in DAB staining on fromalin fixed paraffin-embedded Kidney tissue
  • PAA928Hu01.jpgWB Image
  • Polyclonal Antibody to Tumor Protein p53 (TP53)Figure. Western Blot; Sample: Human MCF7 Cells.
  • CertificateISO9001: 2008, ISO13485: 2003 Registered

SPECIFITY

The antibody is a rabbit polyclonal antibody raised against TP53. It has been selected for its ability to recognize TP53 in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.5-2ug/ml
Immunocytochemistry in formalin fixed cells: 5-20ug/ml
Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml
Immunohistochemistry in paraffin section: 5-20ug/ml
Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Reference

  • Anticancer ResearchAnalysis of p53 and miRNA expression after irradiation of glioblastoma cell linesPubMed: 23155233
  • Microscopy Research and TechniqueTherapeutic role of curcumin in oxidative DNA damage caused by formaldehydePubMed: 25761397
  • J Nutr BiochemSupplementation with Selenium yeast on the prooxidant–antioxidant activities and anti-tumor effects in breast tumor xenograft-bearing micePubMed: 26344777