• KO-Validated
Polyclonal Antibody to Tumor Protein p53 (P53) Homo sapiens (Human) Polyclonal antibody

TP53; LFS1; TRP53; Li-Fraumeni Syndrome; Cellular tumor antigen p53; Antigen NY-CO-13; Phosphoprotein p53; Tumor suppressor p53

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Overview
Properties
  • SourcePolyclonal antibody preparation, Host Rabbit
  • Ig Type IgG, Potency n/a
  • PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
  • LabelNone
  • Immunogen RPA928Hu01-Recombinant Tumor Protein p53 (P53)
  • Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
  • TraitsLiquid, Concentration 0.5mg/ml
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  • Polyclonal Antibody to Tumor Protein p53 (P53) Packages (Simulation)
  • Polyclonal Antibody to Tumor Protein p53 (P53) Packages (Simulation)
  • Polyclonal Antibody to Tumor Protein p53 (P53) FITC staining on IF; Samples: Human HepG2 cell; Primary Ab: 20μg/ml Rabbit Anti-Human TP53 Antibody Second Ab: 1.5μg/ml FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb18)
  • Polyclonal Antibody to Tumor Protein p53 (P53) DAB staining on IHC-P;
    Sample: Porcine Colon Tissue
    Primary Ab: 10µg/ml Rabbit Anti-Human P53 Antibody
    Control: Used PBS instead of primary antibody
    Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Tumor Protein p53 (P53) DAB staining on IHC-P;
    Sample: Porcine Lymph node Tissue
    Primary Ab: 10µg/ml Rabbit Anti-Human P53 Antibody
    Control: Used PBS instead of primary antibody
    Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • PAA928Hu01.jpg Figure 1. Western Blot; Sample: Recombinant TP53, Human.
  • Polyclonal Antibody to Tumor Protein p53 (P53) Western Blot; Sample: Lane1: Rat Spleen lysate; Lane2: Human 293T cell lysate
    Primary Ab: 1µg/mL Rabbit Anti-Human TP53 Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Tumor Protein p53 (P53) Knockout Varification:
    Lane 1: Wild-type MCF7 cell lysate;
    Lane 2: TP53 knockout MCF7 cell lysate;
    Predicted MW: 24,30,33,34,38,39,44kDa
    Observed MW: 50kDa
    Primary Ab: 1µg/ml Rabbit Anti-Human TP53 Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Tumor Protein p53 (P53) Western Blot; Sample: Lane1: A431 cell lysate; Lane2: MCF7 cell lysate
    Primary Ab: 0.3μg/ml Rabbit Anti-Human TP53 Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Tumor Protein p53 (P53) Western Blot; Sample: Porcine Skin lysate
    Primary Ab: 0.3μg/ml Rabbit Anti-Human TP53 Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against P53. It has been selected for its ability to recognize P53 in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.01-2µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Organism Species More: Mus musculus (Mouse), Rattus norvegicus (Rat), Sus scrofa; Porcine (Pig)

Giveaways

Citations

  • Analysis of p53 and miRNA expression after irradiation of glioblastoma cell linesPubMed: 23155233
  • Therapeutic role of curcumin in oxidative DNA damage caused by formaldehydePubMed: 25761397
  • Supplementation with Selenium yeast on the prooxidant–antioxidant activities and anti-tumor effects in breast tumor xenograft-bearing micePubMed: 26344777
  • Transcription factor HBP1: A regulator of senescence and apoptosis of preadipocytesPubmed: 31331641
  • Effect of Graviola (Annona Muricata l.) and Ginger (Zingiber Officinale Roscoe) on Diabetes Mellitus Induced in Male Wistar Albino Rats
  • Non-POU Domain-Containing Octamer-Binding (NONO) Protein Stability Regulated by PIN1 is Crucial for Breast Cancer Tumorigenicity Via the MAPK/β …

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