Polyclonal Antibody to Hypoxanthine Phosphoribosyltransferase 1 (HPRT1)

HGPRT; HGPRTase; HPRT; Lesch-Nyhan Syndrome; Hypoxanthine-guanine phosphoribosyltransferase
Western Blot

IMMUNOGEN INFORMATIONImmunogen: Recombinant HPRT1 (Thr3~Ala218) expressed in E.coli.
Accession No.: RPA717Hu01
Sequence: The target protein is fused with two N-terminal Tags, His-tag and GST-tag and its sequence is listed below.
MRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQGPLGSEF-TRSPGVVI SDDEPGYDLD LFCIPNHYAE DLERVFIPHG LIMDRTERLA RDVMKEMGGH HIVALCVLKG GYKFFADLLD YIKALNRNSD RSIPMTVDFI RLKSYCNDQS TGDIKVIGGD DLSTLTGKNV LIVEDIIDTG KTMQTLLSLV RQYNPKMVKV ASLLVKRTPR SVGYKPDFVG FEIPDKFVVG YALDYNEYFR DLNHVCVISE TGKAKYKA
ANTIBODY SPECIFITY The antibody is a rabbit polyclonal antibody raised against HPRT1. It has been selected for its ability to recognize HPRT1 in immunohistochemical staining and western blotting. APPLICATIONS WB, ICC, IHC-P, IHC-F, ELISA
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.
CONTENTS Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol. QUALITY CONTROL Content: The quality control contains recombinant HPRT1 (Thr3~Ala218) disposed in loading buffer.
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
                5uL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control. Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol, BPB 0.01%, NaN3 0.02%.
STORAGE Store at 4oC for frequent use. Stored at -20oC to -80oC in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Leave your messages here

Welcome to our website. If you have any questions, please send a message to us.
We'll Feedback to you within 24 hours.Our work time is from AM 8:00 to PM 4:30 (U.S. Central Time)

  • Sales:sales@cloud-clone.us
  • After-sales:service@cloud-clone.us
  • Email:mail@cloud-clone.us
  • Close
    Polyclonal Antibody to Hypoxanthine Phosphoribosyltransferase 1 (HPRT1) Material Safety Data Sheet International Organization for Standardization guarantee