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ITEM PROTEIN ANTIBODY ASSAY KIT CELL
Polyclonal Antibody to Lactate Dehydrogenase A (LDHA)
LDH-A; LDH-M; LDH1; PIG19
Organism species Homo sapiens (Human)
Product No.PAB370Hu01
ClonalityPolyclonal
HostRabbit
Immunoglobulin TypeIgG
PurificationAffinity Chromatography
ApplicationsWB, ICC, IHC-P, IHC-F, ELISA
Concentration200ug/ml
UOM100ug
ConjugateNo Conjugate
IMMUNOGEN INFORMATION
Immunogen: Recombinant LDHA (Met1~Phe332) expressed in E.coli.
Accession No.: RPB370Hu01
Sequence: The target protein is fused with a His-tag and its sequence is listed below.
MGHHHHHHSGSEF-MATLKDQLIY NLLKEEQTPQ NKITVVGVGA VGMACAISIL MKDLADELAL VDVIEDKLKG EMMDLQHGSL FLRTPKIVSG KDYNVTANSK LVIITAGARQ QEGESRLNLV QRNVNIFKFI IPNVVKYSPN CKLLIVSNPV DILTYVAWKI SGFPKNRVIG SGCNLDSARF RYLMGERLGVHPLSCHGWVL GEHGDSSVPV WSGMNVAGVS LKTLHPDLGT DKDKEQWKEV HKQVVESAYE VIKLKGYTSW AIGLSVADLA ESIMKNLRRV HPVSTMIKGL YGIKDDVFLS VPCILGQNGI SDLVKVTLTS EEEARLKKSA DTLWGIQKEL QF
ANTIBODY SPECIFITY
The antibody is a rabbit polyclonal antibody raised against LDHA. It has been selected for its ability to recognize LDHA in immunohistochemical staining and western blotting.
APPLICATIONS
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.
CONTENTS
Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
QUALITY CONTROL
Content: The quality control contains recombinant LDHA (Met1~Phe332) disposed in loading buffer.
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
                5uL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control. Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol, BPB 0.01%, NaN3 0.02%.
STORAGE
Store at 4oC for frequent use. Stored at -20oC to -80oC in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Package and Components
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Benchmark price
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications
MAB370Hu22Monoclonal Antibody to Lactate Dehydrogenase A (LDHA)WB, ICC, IHC-P, IHC-F, ELISA
PAB370Hu01Polyclonal Antibody to Lactate Dehydrogenase A (LDHA)WB, ICC, IHC-P, IHC-F, ELISA
PAB370Hu71Biotin-Linked Antibody to Lactate Dehydrogenase A (LDHA)IHC;WB;ELISA
PAB370Hu81FITC-Linked Antibody to Lactate Dehydrogenase A (LDHA)IHC;ICC;IF;WB
RPB370Hu01Recombinant Lactate Dehydrogenase A (LDHA)SDS-PAGE; WB; ELISA; IP.
SEB370HuELISA Kit for Lactate Dehydrogenase A (LDHA)Enzyme-linked immunosorbent assay