Polyclonal Antibody to Lactate Dehydrogenase B (LDHB) Homo sapiens (Human) Polyclonal antibody

LDH-B; LDH-H; Renal carcinoma antigen NY-REN-46

  • Polyclonal Antibody to Lactate Dehydrogenase B (LDHB) Packages (Simulation)
  • Polyclonal Antibody to Lactate Dehydrogenase B (LDHB) Packages (Simulation)
  • Polyclonal Antibody to Lactate Dehydrogenase B (LDHB) IHC Images
  • PAB698Hu01.jpg Figure. Western Blot; Sample: Recombinant LDHB, Human.
  • Polyclonal Antibody to Lactate Dehydrogenase B (LDHB) Figure. Western Blot; Sample. Lane1: Human Jurkat Cells; Lane2: Human A431 Cells; Lane3: Mouse Testis Tissue; Lane4: Mouse Kidney Tissue; Lane5: Human Liver Tissue.
  • Polyclonal Antibody to Lactate Dehydrogenase B (LDHB) Knockout Varification:
    Lane 1: Wild-type Jurkat cell lysate;
    Lane 2: LDHB knockout Jurkat cell lysate;
    Predicted MW: 37kDa
    Observed MW: 35kDa
    Primary Ab: 1µg/ml Rabbit Anti-Human LDHB Ab
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

SPECIFITY

The antibody is a rabbit polyclonal antibody raised against LDHB. It has been selected for its ability to recognize LDHB in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.5-2ug/ml
Immunocytochemistry in formalin fixed cells: 5-20ug/ml
Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml
Immunohistochemistry in paraffin section: 5-20ug/ml
Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Reference

  • Proteome ScienceProteomic analysis of effluents from perfused human heart for transplantation: identification of potential biomarkers for ischemic heart damagProteomesci: 14775956
  • PLoS OnePresence in the Pre-Surgical Fine-Needle Aspiration of Potential Thyroid Biomarkers Previously Identified in the Post-Surgical OnePubMed: PMC3765839