Polyclonal Antibody to Macrophage Migration Inhibitory Factor (MIF) Homo sapiens (Human) Polyclonal antibody

GIF; GLIF; MMIF; Glycosylation-Inhibiting Factor; L-dopachrome isomerase; L-dopachrome tautomerase; Phenylpyruvate tautomerase

  • Product No.PAA698Hu01
  • Organism SpeciesHomo sapiens (Human)
  • SourcePolyclonal antibody preparation
  • HostRabbit
  • Potencyn/a
  • Ig Type IgG
  • PurificationAntigen-specific affinity chromatography
  • LabelNone
  • Immunogen n/a
  • Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
  • TraitsLiquid
  • Concentration200ug/ml
  • Organism Species Moren/a
  • ApplicationsWB, ICC, IHC-P, IHC-F, ELISA
  • DownloadInstruction Manual
  • UOM 10ug 50ug100ug 1mg 1g
  • FOB US$ 95 US$ 221US$ 316 US$ 1264 US$ 94800
  • Polyclonal Antibody to Macrophage Migration Inhibitory Factor (MIF) Package and Components
  • Polyclonal Antibody to Macrophage Migration Inhibitory Factor (MIF) Package and Components
  • Polyclonal Antibody to Macrophage Migration Inhibitory Factor (MIF) Used in DAB staining on fromalin fixed paraffin- embedded liver tissue
  • PAA698Hu01.jpg WB Image
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

SPECIFITY

The antibody is a rabbit polyclonal antibody raised against MIF. It has been selected for its ability to recognize MIF in immunohistochemical staining and western blotting.

USAGE

Western blotting: 1:50-400
Immunocytochemistry in formalin fixed cells: 1:50-500
Immunohistochemistry in formalin fixed frozen section: 1:50-500
Immunohistochemistry in paraffin section: 1:10-100
Enzyme-linked Immunosorbent Assay: 1:100-1:5000
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Reference

  • Kidney International Targeted reduction of advanced glycation improves renal function in obesityPubMed: 21412218
  • The Journal of ImmunologyMacrophage Migration Inhibitory Factor Plays a Role in the Regulation of Microfold (M) Cell-Mediated Transport in the GutJimmunol: 5673
  • The Journal of EndocrinologyInvolvement of exercise-induced macrophage migration inhibitory factor in the prevention of fatty liver diseasePubMed: PMC3757527
  • The Journal of ImmunologyRole of macrophage migration inhibitory factor in the regulatory T cell response of tumor-bearing micePubMed: PMC3466372
  • DiabetologiaDeletion of bone-marrow-derived receptor for AGEs (RAGE) improves renal function in an experimental mouse model of diabetesPubmed:24957662
  • BioMed Research International The Potential Role of Polymethyl Methacrylate as a New Packaging Material for the Implantable Medical Device in the BladderPubMed: 25705692