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ITEM PROTEIN ANTIBODY ASSAY KIT CELL
Polyclonal Antibody to Macrophage Migration Inhibitory Factor (MIF)
GIF; GLIF; MMIF; Glycosylation-Inhibiting Factor
Organism species Homo sapiens (Human)
Product No.PAA698Hu01
ClonalityPolyclonal
HostRabbit
Immunoglobulin TypeIgG
PurificationAffinity Chromatography
ApplicationsWB, ICC, IHC-P, IHC-F, ELISA
Concentration200ug/ml
UOM100ug
ConjugateNo Conjugate
IMMUNOGEN INFORMATION
Immunogen: Recombinant MIF (Met3~Asn111) expressed in E.coli.
Accession No.: RPA698Hu01
Sequence: The target protein is fused with N-terminal His-Tag and its sequence is listed below.
MGHHHHHHSGSEF- MFIVNTNV PRASVPDGFL SELTQQLAQA TGKPPQYIAV HVVPDQLMAF GGSSEPCALC SLHSIGKIGG AQNRSYSKLL CGLLAERLRI SPDRVYINYY DMNAANVGWN N
ANTIBODY SPECIFITY
The antibody is a rabbit polyclonal antibody raised against MIF. It has been selected for its ability to recognize MIF in immunohistochemical staining and western blotting.
APPLICATIONS
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.
CONTENTS
Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
QUALITY CONTROL
Content: The quality control contains recombinant MIF (Met3~Asn111) disposed in loading buffer.
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
                5uL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control. Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol, BPB 0.01%, NaN3 0.02%.
STORAGE
Store at 4oC for frequent use. Stored at -20oC to -80oC in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Package and Components
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Benchmark price
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications
MAA698Hu22Monoclonal Antibody to Macrophage Migration Inhibitory Factor (MIF)WB, ICC, IHC-P, IHC-F, ELISA
PAA698Hu01Polyclonal Antibody to Macrophage Migration Inhibitory Factor (MIF)WB, ICC, IHC-P, IHC-F, ELISA
PAA698Hu02Polyclonal Antibody to Macrophage Migration Inhibitory Factor (MIF)WB, ICC, IHC-P, IHC-F, ELISA
RPA698Hu01Recombinant Macrophage Migration Inhibitory Factor (MIF)SDS-PAGE; WB; ELISA; IP.
RPA698Hu02Recombinant Macrophage Migration Inhibitory Factor (MIF)SDS-PAGE; WB; ELISA; IP.
SEA698HuELISA Kit for Macrophage Migration Inhibitory Factor (MIF)Enzyme-linked immunosorbent assay