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ITEM PROTEIN ANTIBODY ASSAY KIT CELL
Polyclonal Antibody to Matrix Metalloproteinase 10 (MMP10)
STMY2; SL2; Stromelysin 2
Organism species Homo sapiens (Human)
Product No.PAA098Hu01
ClonalityPolyclonal
HostRabbit
Immunoglobulin TypeIgG
PurificationAffinity Chromatography
ApplicationsWB, ICC, IHC-P, IHC-F, ELISA
Concentration200ug/ml
UOM100ug
ConjugateNo Conjugate
IMMUNOGEN INFORMATION
Immunogen: Recombinant MMP10 (Phe99~Cys476) expressed in E.coli.
Accession No.: RPA098Hu01
Sequence: The target protein is fused with a His-tag and its sequence is listed below.
MGHHHHHHSGSEF-FS SFPGMPKWRK THLTYRIVNY TPDLPRDAVD SAIEKALKVW EEVTPLTFSR LYEGEADIMI SFAVKEHGDF YSFDGPGHSL AHAYPPGPGL YGDIHFDDDE KWTEDASGTN LFLVAAHELG HSLGLFHSAN TEALMYPLYN SFTELAQFRL SQDDVNGIQS LYGPPPASTE EPLVPTKSVP SGSEMPAKCD PALSFDAIST LRGEYLFFKD RYFWRRSHWN PEPEFHLISA FWPSLPSYLD AAYEVNSRDT VFIFKGNEFW AIRGNEVQAG YPRGIHTLGF PPTIRKIDAA VSDKEKKKTY FFAADKYWRF DENSQSMEQG FPRLIADDFP GVEPKVDAVL QAFGFFYFFS GSSQFEFDPN ARMVTHILKS NSWLHC
ANTIBODY SPECIFITY
The antibody is a rabbit polyclonal antibody raised against MMP10. It has been selected for its ability to recognize MMP10 in immunohistochemical staining and western blotting.
APPLICATIONS
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.
CONTENTS
Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
QUALITY CONTROL
Content: The quality control contains recombinant Thyroglobulin (Ser2083~Ala2333) disposed in loading buffer.
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
                5uL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control. Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol, BPB 0.01%, NaN3 0.02%.
STORAGE
Store at 4oC for frequent use. Stored at -20oC to -80oC in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Package and Components
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Benchmark price
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications
MAA098Hu22Monoclonal Antibody to Matrix Metalloproteinase 10 (MMP10)WB, ICC, IHC-P, IHC-F, ELISA
PAA098Hu01Polyclonal Antibody to Matrix Metalloproteinase 10 (MMP10)WB, ICC, IHC-P, IHC-F, ELISA
PAA098Hu71Biotin-Linked Antibody to Matrix Metalloproteinase 10 (MMP10)IHC;WB;ELISA
PAA098Hu81FITC-Linked Antibody to Matrix Metalloproteinase 10 (MMP10)IHC;ICC;IF;WB
RPA098Hu01Recombinant Matrix Metalloproteinase 10 (MMP10)SDS-PAGE; WB; ELISA; IP.
SCA098HuCLIA Kit for Matrix Metalloproteinase 10 (MMP10)Chemiluminescent immunoassay
SEA098HuELISA Kit for Matrix Metalloproteinase 10 (MMP10)Enzyme-linked immunosorbent assay