Polyclonal Antibody to Milk Fat Globule EGF Factor 8 (MFGE8) Mus musculus (Mouse) Polyclonal antibody

HMFG; MFGM; SED1; BA46; EDIL1; OAcGD3S; SPAG10; hP47; Medin; Lactadherin; Sperm Associated Antigen 10; Sperm Surface Protein hP47; Milk fat globule-EGF factor 8

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Overview
Properties
  • SourcePolyclonal antibody preparation, Host Rabbit
  • Ig Type IgG, Potency n/a
  • PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
  • LabelNone
  • Immunogen RPB286Mu01-Recombinant Milk Fat Globule EGF Factor 8 (MFGE8)
  • Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
  • TraitsLiquid, Concentration 500µg/mL
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  • Polyclonal Antibody to Milk Fat Globule EGF Factor 8 (MFGE8) Packages (Simulation)
  • Polyclonal Antibody to Milk Fat Globule EGF Factor 8 (MFGE8) Packages (Simulation)
  • Polyclonal Antibody to Milk Fat Globule EGF Factor 8 (MFGE8) IHC Images
  • PAB286Mu01.jpg Figure. Western Blot; Sample: Recombinant MFGE8, Mouse.
  • Polyclonal Antibody to Milk Fat Globule EGF Factor 8 (MFGE8) Western Blot; Sample: Mouse Cerebrum lysate;
    Primary Ab: 5µg/ml Rabbit Anti-Mouse MFGE8 Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Milk Fat Globule EGF Factor 8 (MFGE8) Western Blot; Sample: Mouse Heart lysate;
    Primary Ab: 5µg/ml Rabbit Anti-Mouse MFGE8 Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Milk Fat Globule EGF Factor 8 (MFGE8) Western Blot; Sample: Mouse Kidney lysate;
    Primary Ab: 5µg/ml Rabbit Anti-Mouse MFGE8 Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against MFGE8. It has been selected for its ability to recognize MFGE8 in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.5-5µg/mL;
Immunohistochemistry: 5-50µg/mL;
Immunocytochemistry: 5-50µg/mL;
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Giveaways

Citations

  • Tumor necrosis factor α up-regulates endometrial milk fat globule-epidermal growth factor 8 protein production via nuclear factor κB activation, resulting in cell migration of epithelial cells.Pubmed: 24262600
  • Biomarker for amyloid-b-related neurological disordersUS20130302838
  • Elevated serum milk fat globule-epidermal growth factor 8 levels in type 2 diabetic patients are suppressed by overweight or obese status.pubmed:28067023
  • Circulating milk fat globule-epidermal growth factor 8 levels are increased in pregnancy and gestational diabetes mellitus.pubmed:28035763
  • Modifications of Western-type diet regarding protein, fat and sucrose levels as modulators of steroid metabolism and activity in liverpubmed:27471150
  • Depressive-like effect of prenatal exposure to DDT involves global DNA hypomethylation and impairment of GPER1/ESR1 protein levels but not ESR2 and AHR/ARNT signalingpubmed:28263910
  • Pantoprazole blocks the JAK2/STAT3 pathway to alleviate skeletal muscle wasting in cancer cachexia by inhibiting inflammatory response.pubmed:28489606
  • TGF-β1 enhances phagocytic removal of neuron debris and neuronal survival by olfactory ensheathing cells via integrin/MFG-E8 signaling pathway10.1016/j.mcn.2017.08.006
  • Needle-shaped amyloid deposition in rat mammary gland: evidence of a novel amyloid fibril proteinPubmed: 31615282
  • Milk fat globule EGF factor 8 restores mitochondrial function via integrin©\medicated activation of the FAK©\STAT3 signaling pathway in acute pancreatitis33634976

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