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ITEM PROTEIN ANTIBODY ASSAY KIT CELL
Polyclonal Antibody to Mitogen Activated Protein Kinase 8 (MAPK8)
JNK1; JNK1A2; JNK21B1/2; PRKM8; SAPK1; JUN N-Terminal Kinase
Organism species Homo sapiens (Human)
Product No.PAB156Hu01
ClonalityPolyclonal
HostRabbit
Immunoglobulin TypeIgG
PurificationAffinity Chromatography
ApplicationsWB, ICC, IHC-P, IHC-F, ELISA
Concentration200ug/ml
UOM100ug
ConjugateNo Conjugate
IMMUNOGEN INFORMATION
Immunogen: Recombinant MAPK8 (Ala145~Ala419) expressed in E.coli.
Accession No.: RPB156Hu01
Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGS-AGIIHR DLKPSNIVVK SDCTLKILDF GLARTAGTSF MMTPYVVTRY YRAPEVILGM GYKENVDLWS VGCIMGEMVC HKILFPGRDY IDQWNKVIEQ LGTPCPEFMK KLQPTVRTYV ENRPKYAGYS FEKLFPDVLF PADSEHNKLK ASQARDLLSK MLVIDASKRI SVDEALQHPY INVWYDPSEA EAPPPKIPDK QLDEREHTIE EWKELIYKEV MDLEERTKNG VIRGQPSPLG AAVINGSQHP SSSSSVNDVS SMSTDPTLAS DTDSSLEAA
ANTIBODY SPECIFITY
The antibody is a rabbit polyclonal antibody raised against MAPK8. It has been selected for its ability to recognize MAPK8 in immunohistochemical staining and western blotting.
APPLICATIONS
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.
CONTENTS
Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
QUALITY CONTROL
Content: The quality control contains recombinant MAPK8 (Ala145~Ala419) disposed in loading buffer.
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
                5uL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control. Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol, BPB 0.01%, NaN3 0.02%.
STORAGE
Store at 4oC for frequent use. Stored at -20oC to -80oC in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Package and Components
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Benchmark price
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications
PAB156Hu01Polyclonal Antibody to Mitogen Activated Protein Kinase 8 (MAPK8)WB, ICC, IHC-P, IHC-F, ELISA
RPB156Hu01Recombinant Mitogen Activated Protein Kinase 8 (MAPK8)SDS-PAGE; WB; ELISA; IP.