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ITEM PROTEIN ANTIBODY ASSAY KIT CELL
Polyclonal Antibody to Mitogen Activated Protein Kinase Kinase 1 (MAP2K1)
MAPKK1; MEK1; MKK1; PRKMK1
Organism species Homo sapiens (Human)
Product No.PAD559Hu01
ClonalityPolyclonal
HostRabbit
Immunoglobulin TypeIgG
PurificationAffinity Chromatography
ApplicationsWB, ICC, IHC-P, IHC-F, ELISA
Concentration200ug/ml
UOM100ug
ConjugateNo Conjugate
IMMUNOGEN INFORMATION
Immunogen: Recombinant MAP2K1 (Leu54~Val369) expressed in E.coli.
Accession No.: RPD559Hu01
Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- LTQKQKV GELKDDDFEK ISELGAGNGG VVFKVSHKPS GLVMARKLIH LEIKPAIRNQ IIRELQVLHE CNSPYIVGFY GAFYSDGEIS ICMEHMDGGS LDQVLKKAGR IPEQILGKVS IAVIKGLTYL REKHKIMHRD VKPSNILVNS RGEIKLCDFG VSGQLIDSMA NSFVGTRSYM SPERLQGTHY SVQSDIWSMG LSLVEMAVGR YPIPPPDAKE LELMFGCQVE GDAAETPPRP RTPGRPLSSY GMDSRPPMAI FELLDYIVNE PPPKLPSGVF SLEFQDFVNK CLIKNPAERA DLKQLMVHAF IKRSDAEEV
ANTIBODY SPECIFITY
The antibody is a rabbit polyclonal antibody raised against MAP2K1. It has been selected for its ability to recognize MAP2K1 in immunohistochemical staining and western blotting.
APPLICATIONS
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.
CONTENTS
Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
QUALITY CONTROL
Content: The quality control contains recombinant Thyroglobulin (Ser2083~Ala2333) disposed in loading buffer.
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
                5uL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control. Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol, BPB 0.01%, NaN3 0.02%.
STORAGE
Store at 4oC for frequent use. Stored at -20oC to -80oC in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Package and Components
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Benchmark price
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications
MAD559Hu21Monoclonal Antibody to Mitogen Activated Protein Kinase Kinase 1 (MAP2K1)WB, ICC, IHC-P, IHC-F, ELISA
PAD559Hu01Polyclonal Antibody to Mitogen Activated Protein Kinase Kinase 1 (MAP2K1)WB, ICC, IHC-P, IHC-F, ELISA
RPD559Hu01Recombinant Mitogen Activated Protein Kinase Kinase 1 (MAP2K1)SDS-PAGE; WB; ELISA; IP.
SED559HuELISA Kit for Mitogen Activated Protein Kinase Kinase 1 (MAP2K1)Enzyme-linked immunosorbent assay