Home  |   About us
ITEM PROTEIN ANTIBODY ASSAY KIT CELL
Polyclonal Antibody to Nitric Oxide Synthase 1, Neuronal (NOS1)
NOS; nNOS; IHPS1; Nitric Ocide Synthase
Organism species Homo sapiens (Human)
Product No.PAA815Hu01
ClonalityPolyclonal
HostRabbit
Immunoglobulin TypeIgG
PurificationAffinity Chromatography
ApplicationsWB, ICC, IHC-P, IHC-F, ELISA
Concentration200ug/ml
UOM100ug
ConjugateNo Conjugate
IMMUNOGEN INFORMATION
Immunogen: Recombinant NOS1 (Ile1199~Ile1399) expressed in E.coli.
Accession No.: RPA815Hu01
Sequence: The target protein is fused with N-terminal His-Tag and its sequence is listed below.
MGHHHHHHSG S- IV SYRTRDGEGP IHHGVCSSWL NRIQADELVP CFVRGAPSFH LPRNPQVPCI LVGPGTGIAP FRSFWQQRQF DIQHKGMNPC PMVLVFGCRQ SKIDHIYREE TLQAKNKGVF RELYTAYSRE PDKPKKYVQD ILQEQLAESV YRALKEQGGH IYVCGDVTMA ADVLKAIQRI MTQQGKLSAE DAGVFISRMR DDNRYHEDI
ANTIBODY SPECIFITY
The antibody is a rabbit polyclonal antibody raised against NOS1. It has been selected for its ability to recognize NOS1 in immunohistochemical staining and western blotting.
APPLICATIONS
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.
CONTENTS
Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
QUALITY CONTROL
Content: The quality control contains recombinant Thyroglobulin (Ser2083~Ala2333) disposed in loading buffer.
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
                5uL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control. Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol, BPB 0.01%, NaN3 0.02%.
STORAGE
Store at 4oC for frequent use. Stored at -20oC to -80oC in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Package and Components
Download
Benchmark price
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications
MAA815Hu22Monoclonal Antibody to Nitric Oxide Synthase 1, Neuronal (NOS1)WB, ICC, IHC-P, IHC-F, ELISA
PAA815Hu01Polyclonal Antibody to Nitric Oxide Synthase 1, Neuronal (NOS1)WB, ICC, IHC-P, IHC-F, ELISA
RPA815Hu01Recombinant Nitric Oxide Synthase 1, Neuronal (NOS1)SDS-PAGE; WB; ELISA; IP.
SCA815HuCLIA Kit for Nitric Oxide Synthase 1, Neuronal (NOS1)Chemiluminescent immunoassay
SEA815HuELISA Kit for Nitric Oxide Synthase 1, Neuronal (NOS1)Enzyme-linked immunosorbent assay