• KO-Validated
Polyclonal Antibody to Prosaposin (PSAP) Homo sapiens (Human) Polyclonal antibody

SAP1; GLBA; CSAct; Dispersin; Pro-Saposin; Proactivator polypeptide; Glucosylceramidase activator; Sulfatide/GM1 activator; Cerebroside sulfate activator; Sphingolipid Activator 1

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Overview
Properties
  • SourcePolyclonal antibody preparation, Host Rabbit
  • Ig Type IgG, Potency n/a
  • PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
  • LabelNone
  • Immunogen RPC756Hu01-Recombinant Prosaposin (PSAP)
  • Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
  • TraitsLiquid, Concentration 500µg/mL
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  • Polyclonal Antibody to Prosaposin (PSAP) Packages (Simulation)
  • Polyclonal Antibody to Prosaposin (PSAP) Packages (Simulation)
  • Polyclonal Antibody to Prosaposin (PSAP) DAB staining on IHC-P; Samples: Human Cerebrum Tissue; Primary Ab: 10µg/ml Rabbit Anti-Human PSAP Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Prosaposin (PSAP) DAB staining on IHC-P;
    Samples: Human Glioma Tissue;
    Primary Ab: 10µg/ml Rabbit Anti-Human PSAP Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Prosaposin (PSAP) DAB staining on IHC-P;
    Samples: Human Liver Tissue;
    Primary Ab: 10µg/ml Rabbit Anti-Human PSAP Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Prosaposin (PSAP) DAB staining on IHC-P;
    Sample: Rat Spinal cord Tissue;
    Primary Ab: 20ug/ml Rabbit Anti-Human PSAP Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Prosaposin (PSAP) DAB staining on IHC-P;
    Sample: Rat Liver Tissue;
    Primary Ab: 20ug/ml Rabbit Anti-Human PSAP Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Prosaposin (PSAP) DAB staining on IHC-P;
    Sample: Rat Uterus Tissue;
    Primary Ab: 20ug/ml Rabbit Anti-Human PSAP Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Prosaposin (PSAP) Vector Red staining on IHC-P;
    Samples: Human Spleen Tissue;
    Primary Ab: 10µg/ml Rabbit Anti-Human PSAP Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
  • Polyclonal Antibody to Prosaposin (PSAP) Figure. FITC staining on IHC-P; Samples: Human Hela Cells.
  • Polyclonal Antibody to Prosaposin (PSAP) FITC staining on IF;
    Sample: Human HepG2 cell;
    Primary Ab: 20ug/ml Rabbit Anti-Human PSAP Antibody
    Second Ab: 2µg/ml FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb18)
  • PAC756Hu01.jpg Figure. Western Blot ; Sample: Recombinant PSAP, Human.
  • Polyclonal Antibody to Prosaposin (PSAP) Western Blot; Sample: Lane1: Porcine Skin lysate; Lane2: A431 cell lysate; Lane3: 293T cell lysate; Lane4: Hela cell lysate
    Primary Ab: 0.1µg/ml Rabbit Anti-Human PSAP Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Prosaposin (PSAP) Knockout Varification:
    Lane 1: Wild-type 293T cell lysate;
    Lane 2: PSAP knockout 293T cell lysate;
    Predicted MW: 58kd
    Observed MW: 60kd
    Primary Ab: 2µg/ml Rabbit Anti-Human PSAP Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against PSAP. It has been selected for its ability to recognize PSAP in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.01-2µg/mL
Immunohistochemistry: 5-20µg/mL
Immunocytochemistry: 5-20µg/mL
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Organism Species More: Rattus norvegicus (Rat), Sus scrofa; Porcine (Pig)

Giveaways

Citations

  • Testican 1 (SPOCK1) and protein tyrosine phosphatase, receptor type S (PTPRS) show significant increase in saliva of tobacco users with oral cancerDoi: 10.1177/2057178X18800534
  • Prosaposin in seminal plasma on the day of oocyte retrieval is associated with normal fertilization and embryo development in in vitro fertilization cyclesPubmed: 31844578
  • Putative Biomarkers for Malignant Pleural Mesothelioma Suggested by Proteomic Analysis of Cell SecretomePubmed: 32345664
  • Surface Protein Dispersin of Enteroaggregative Escherichia coli Binds Plasminogen That Is Converted Into Active PlasminPubmed: 32625178

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