Monoclonal Antibody to Tumor Protein p53 (P53) Homo sapiens (Human) Monoclonal antibody

TP53; LFS1; TRP53; Li-Fraumeni Syndrome; Cellular tumor antigen p53; Antigen NY-CO-13; Phosphoprotein p53; Tumor suppressor p53

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Overview
Properties
  • SourceMonoclonal antibody preparation, Host Mouse
  • Ig Isotype IgG1 Kappa, Clone Number D8
  • PurificationProtein A + Protein G affinity chromatography
  • LabelNone
  • Immunogen RPA928Hu03-Recombinant Tumor Protein p53 (P53)
  • Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
  • TraitsLiquid, Concentration 1mg/ml
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  • Monoclonal Antibody to Tumor Protein p53 (P53) Packages (Simulation)
  • Monoclonal Antibody to Tumor Protein p53 (P53) Packages (Simulation)
  • MAA928Hu22.jpg Western Blot; Sample: Lane1: A431 cell lysate; Lane2: MCF7 cell lysate; Lane3: 293T cell lysate Primary Ab: 2μg/ml Mouse Anti-Human TP53 Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a mouse monoclonal antibody raised against P53. It has been selected for its ability to recognize P53 in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.01-2µg/mL;
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Giveaways

Citations

  • Analysis of p53 and miRNA expression after irradiation of glioblastoma cell linesPubMed: 23155233
  • Therapeutic role of curcumin in oxidative DNA damage caused by formaldehydePubMed: 25761397
  • Supplementation with Selenium yeast on the prooxidant–antioxidant activities and anti-tumor effects in breast tumor xenograft-bearing micePubMed: 26344777
  • Transcription factor HBP1: A regulator of senescence and apoptosis of preadipocytesPubmed: 31331641
  • Effect of Graviola (Annona Muricata l.) and Ginger (Zingiber Officinale Roscoe) on Diabetes Mellitus Induced in Male Wistar Albino Rats
  • Non-POU Domain-Containing Octamer-Binding (NONO) Protein Stability Regulated by PIN1 is Crucial for Breast Cancer Tumorigenicity Via the MAPK/β …

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