Polyclonal Antibody to Ubiquitin Carboxyl Terminal Hydrolase L1 (UCHL1) Homo sapiens (Human) Polyclonal antibody

PARK5; PGP9.5; Uch-L1; Neuron cytoplasmic protein 9.5; Ubiquitin thioesterase L1; Ubiquitin carboxyl-terminal hydrolase isozyme L1

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Overview
Properties
  • SourcePolyclonal antibody preparation, Host Rabbit
  • Ig Type IgG, Potency n/a
  • PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
  • LabelNone
  • Immunogen RPG945Hu01-Recombinant Ubiquitin Carboxyl Terminal Hydrolase L1 (UCHL1)
  • Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
  • TraitsLiquid, Concentration 500µg/mL
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  • Polyclonal Antibody to Ubiquitin Carboxyl Terminal Hydrolase L1 (UCHL1) Packages (Simulation)
  • Polyclonal Antibody to Ubiquitin Carboxyl Terminal Hydrolase L1 (UCHL1) Packages (Simulation)
  • Polyclonal Antibody to Ubiquitin Carboxyl Terminal Hydrolase L1 (UCHL1) DAB staining on IHC-P; Samples: Human Kidney Tissue; Primary Ab: 10µg/ml Rabbit Anti-Human UCHL1 Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Ubiquitin Carboxyl Terminal Hydrolase L1 (UCHL1) DAB staining on IHC-P;
    Samples: Human Glioma Tissue;
    Primary Ab: 10µg/ml Rabbit Anti-Human UCHL1 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Ubiquitin Carboxyl Terminal Hydrolase L1 (UCHL1) DAB staining on IHC-P;
    Samples: Human Brain Tissue;
    Primary Ab: 10µg/ml Rabbit Anti-Human UCHL1 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Ubiquitin Carboxyl Terminal Hydrolase L1 (UCHL1) DAB staining on IHC-P;
    Samples: Human Lung cancer Tissue;
    Primary Ab: 10µg/ml Rabbit Anti-Human UCHL1 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Ubiquitin Carboxyl Terminal Hydrolase L1 (UCHL1) DAB staining on IHC-P;
    Samples: Human Prostate cancer Tissue;
    Primary Ab: 10µg/ml Rabbit Anti-Human UCHL1 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Ubiquitin Carboxyl Terminal Hydrolase L1 (UCHL1) DAB staining on IHC-P;
    Samples: Human Liver cancer Tissue;
    Primary Ab: 10µg/ml Rabbit Anti-Human UCHL1 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Ubiquitin Carboxyl Terminal Hydrolase L1 (UCHL1) Figure: FITC staining on IHC-P;
    Sample: A549 cells.
  • PAG945Hu01.jpg Figure. Western Blot; Sample: Recombinant UCHL1, Human.
  • Polyclonal Antibody to Ubiquitin Carboxyl Terminal Hydrolase L1 (UCHL1) Figure. Western Blot; Sample: Lane1: Mouse Brain Tissue; Lane2: Porcine Brain Tissue; Lane3: Human U-87MG Cells; Lane4: Human A549 Cells.
  • Polyclonal Antibody to Ubiquitin Carboxyl Terminal Hydrolase L1 (UCHL1) Human SH-SY5Y cell was fixed with 2% paraformaldehyde (10 min) , permeabilised with 0.1% BSA-Triton X-100,then stained with 20µg/ml rabbit Anti-human UCHL1 Polyclonal Antibody (Catalog PAC030Hu01, red histogram) or Isotype control antibody (Catalog IS067-Rb01, green histogram), followed by 1µg/ml FITC-conjugated Anti-rabbit IgG Secondary Antibody (Catalog SAA544Rb18).
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against UCHL1. It has been selected for its ability to recognize UCHL1 in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.5-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Organism Species More: Mus musculus (Mouse), Rattus norvegicus (Rat), Sus scrofa; Porcine (Pig)

Giveaways

Citations

  • Changes of ubiquitin C-terminal hydrolase-L1 levels in serum and urine of patients with white matter lesionsPubMed: 26232084
  • Increased plasma UCH-L1 after aneurysmal subarachnoid hemorrhage is associated with unfavorable neurological outcomePubmed:26810533
  • Serum Carnosine Dipeptidase 1 and Ubiquitin C - Terminal Hydrolase L1 as Markers of Brain Damage in Patients after Carotid Endarterectomy mnstemps:135
  • Activation of hepatic stellate cells by the ubiquitin C-terminal hydrolase 1 protein secreted from hepatitis C virus-infected hepatocytespubmed:28667290
  • Relationships between markers of neurologic and endothelial injury during critical illness and long-term cognitive impairment and disabilityPubmed:29523900
  • Serum concentration of ubiquitin c-terminal hydrolase-L1 in detecting severity of traumatic brain injuryarticle:10.1088
  • Peripheral blood biomarkers in aneurysmal subarachnoid hemorrhageISBN:978-952-03-0750-9
  • Association of neuronal repair biomarkers with delirium among survivors of critical illnessPubmed: 31896448
  • BDNF and IL-8, But Not UCHL-1 and IL-11, Are Markers of Brain Injury in Children Caused by Mild Head TraumaPubmed: 32987792

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