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ITEM PROTEIN ANTIBODY ASSAY KIT CELL
Polyclonal Antibody to Uromodulin (UMOD)
Uromucoid,Tamm-Horsfall Glycoprotein
Organism species Homo sapiens (Human)
Product No.PAG918Hu01
ClonalityPolyclonal
HostRabbit
Immunoglobulin TypeIgG
PurificationAffinity Chromatography
ApplicationsWB, ICC, IHC-P, IHC-F, ELISA
Concentration200ug/ml
UOM100ug
ConjugateNo Conjugate
IMMUNOGEN INFORMATION
Immunogen: Recombinant UMOD (Glu334~Ser589) expressed in E.coli.
Accession No.: RPG918Hu01
Sequence: The target protein is fused with N-terminal His-Tag and its sequence is listed below.
MGHHHHHHSGS-ECGANDM KVSLGKCQLK SLGFDKVFMY LSDSRCSGFN DRDNRDWVSV VTPARDGPCG TVLTRNETHA TYSNTLYLAD EIIIRDLNIK INFACSYPLD MKVSLKTALQ PMVSALNIRV GGTGMFTVRM ALFQTPSYTQ PYQGSSVTLS TEAFLYVGTM LDGGDLSRFA LLMTNCYATP SSNATDPLKY FIIQDRCPHT RDSTIQVVEN GESSQGRFSV QMFRFAGNYD LVYLHCEVYL CDTMNEKCKP TCSGTRFRS
ANTIBODY SPECIFITY
The antibody is a rabbit polyclonal antibody raised against UMOD. It has been selected for its ability to recognize UMOD in immunohistochemical staining and western blotting.
APPLICATIONS
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.
CONTENTS
Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
QUALITY CONTROL
Content: The quality control contains recombinant UMOD (Glu334~Ser589) disposed in loading buffer.
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
                5uL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control. Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol, BPB 0.01%, NaN3 0.02%.
STORAGE
Store at 4oC for frequent use. Stored at -20oC to -80oC in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Package and Components
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Benchmark price
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications
PAG918Hu01Polyclonal Antibody to Uromodulin (UMOD)WB, ICC, IHC-P, IHC-F, ELISA
PAG918Hu71Biotin-Linked Antibody to Uromodulin (UMOD)IHC;WB;ELISA
RPG918Hu01Recombinant Uromodulin (UMOD)SDS-PAGE; WB; ELISA; IP.
SEG918HuELISA Kit for Uromodulin (UMOD)Enzyme-linked immunosorbent assay