Polyclonal Antibody to Vinculin (VCL)Homo sapiens (Human)Polyclonal antibody

MVCL; Metavinculin

  • Polyclonal Antibody to Vinculin (VCL)Package and Components
  • Polyclonal Antibody to Vinculin (VCL)Package and Components
  • Polyclonal Antibody to Vinculin (VCL)Used in DAB staining on fromalin fixed paraffin-embedded stomach tissue
  • PAB839Hu01.jpgWB Image
  • Polyclonal Antibody to Vinculin (VCL)Figure. Western Blot; Sample. Lane1: Mouse Heart Tissue; Lane2: Human Lung Tissue.
  • CertificateISO9001: 2008, ISO13485: 2003 Registered

SPECIFITY

The antibody is a rabbit polyclonal antibody raised against VCL. It has been selected for its ability to recognize VCL in immunohistochemical staining and western blotting.

USAGE

Western blotting: 0.5-2ug/ml
Immunocytochemistry in formalin fixed cells: 5-20ug/ml
Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml
Immunohistochemistry in paraffin section: 5-20ug/ml
Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml
Optimal working dilutions must be determined by end user.

STORAGE

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Reference

  • PBMCs S-NO proteomic signature in heart failureS-Nitrosylation Proteome Profile of Peripheral Blood Mononuclear Cells in Human Heart Failurejournals:1384523