Polyclonal Antibody to Vinculin (VCL) Mus musculus (Mouse) Polyclonal antibody

MVCL; Metavinculin

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Overview
Properties
  • SourcePolyclonal antibody preparation, Host Rabbit
  • Ig Type IgG, Potency n/a
  • PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
  • LabelNone
  • Immunogen RPB839Mu01-Recombinant Vinculin (VCL)
  • Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
  • TraitsLiquid, Concentration 500µg/mL
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  • Polyclonal Antibody to Vinculin (VCL) Packages (Simulation)
  • Polyclonal Antibody to Vinculin (VCL) Packages (Simulation)
  • Polyclonal Antibody to Vinculin (VCL) DAB staining on IHC-P; Samples: Mouse Heart Tissue; Primary Ab: 20μg/ml Rabbit Anti-Mouse VCL Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Vinculin (VCL) DAB staining on IHC-P;
    Samples: Mouse Kidney Tissue;
    Primary Ab: 20μg/ml Rabbit Anti-Mouse VCL Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Vinculin (VCL) DAB staining on IHC-P;
    Sample: Mouse Cardiac Muscle Tissue
    Primary Ab: 20µg/ml Rabbit Anti-Mouse VCL Antibody
    Control: Used PBS instead of primary antibody
    Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • PAB839Mu01.jpg Figure. Western Blot; Sample: Recombinant VCL, Mouse.
  • Polyclonal Antibody to Vinculin (VCL) Western Blot; Sample:Human Lung lysate;
    Primary Ab: 2µg/ml Rabbit Anti-Mouse VCL Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Vinculin (VCL) Western Blot; Sample: Mouse Heart lysate;
    Primary Ab: 2µg/ml Rabbit Anti-Mouse VCL Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against VCL. It has been selected for its ability to recognize VCL in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.5-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Organism Species More: Homo sapiens (Human), Rattus norvegicus (Rat)

Giveaways

Citations

  • S-Nitrosylation Proteome Profile of Peripheral Blood Mononuclear Cells in Human Heart Failurejournals:1384523
  • PCTAIRE1 promotes mitotic progression and resistance against antimitotic and apoptotic signalsPubmed:35044463

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