Monoclonal Antibody to Visfatin (VF) Homo sapiens (Human) Monoclonal antibody

Nampt; NAmPRTase; PBEF; PBEF1; Pre-B-Cell Colony Enhancing Factor; Nicotinamide Phosphoribosyltransferase

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Overview
Properties
  • SourceMonoclonal antibody preparation, Host Mouse
  • Ig Isotype IgG1 Kappa, Clone Number D1
  • PurificationProtein A + Protein G affinity chromatography
  • LabelNone
  • Immunogen RPA638Hu01-Recombinant Visfatin (VF)
  • Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
  • TraitsLiquid, Concentration 1mg/mL
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  • Monoclonal Antibody to Visfatin (VF) Packages (Simulation)
  • Monoclonal Antibody to Visfatin (VF) Packages (Simulation)
  • Monoclonal Antibody to Visfatin (VF) IHC Images
  • MAA638Hu22.jpg Western Blot; Sample: Lane1: Human Milk; Lane2: Rat Liver lysate; Lane3: Rat Skeletal muscle lysate; Lane4: 293T cell lysate Primary Ab: 2µg/ml Mouse Anti-Human Visfatin Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a mouse monoclonal antibody raised against VF. It has been selected for its ability to recognize VF in immunohistochemical staining and western blotting.

Usage

Western blottin:g: 0.5-5µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Organism Species More: Rattus norvegicus (Rat)

Giveaways

Citations

  • EFFECT OF 8 WEEKS ENDURANCE TRAINING ON PLASMA VISFATIN IN MIDDLE-AGED MENBrjb: source
  • The relationship between visfatin and HOMA-IR in hypertensive patients, and the effect of antihypertensive drugs on visfatin and HOMA-IR in hypertensive patients with insulin resistanceScienceDirect: S0168822711002956
  • Early-effect of bariatric surgery (Scopinaro method) on intestinal hormones and adipokines in insulin resistant Wistar rat.Pubmed: 24304571
  • CTRP3 modulates the expression and secretion of adipokines in 3T3-L1 adipocytesPubmed:25168658
  • Plasma visfatin levels and mRNA expression of visfatin in peripheral blood mononuclear cells and peripheral blood monocyte-derived macrophages from normal weight females with polycystic ovary syndromePubmed:Pmc3991522
  • Visfatin derived from ascites promotes ovarian cancer cell migration through Rho/ROCK signaling-mediated actin polymerization.Pubmed:25055182
  • Visfatin −948G/T and resistin −420C/Gpolymorphisms in Egyptian type 2 diabetic patients with and without cardiovascular diseasesNrcresearchpress:Source
  • Antidiabetic Effect of Galantamine: Novel Effect for a Known Centrally Acting DrugPubMed: 26262991
  • Changes in concentration of visfatin during four weeks of inpatient treatment of alcohol dependent malesScience: Article
  • Variation in blood levels of hormones in obese patients following weight reduction induced by endoscopic and surgical bariatric therapiesPubMed: 26539806
  • Genetic Variations in Visfatin and Resistin Genes in Egyptian Type 2 Diabetic Patients With and Without ComplicationsPubMed: 25120107
  • Favorable outcomes of hydroxychloroquine in insulin resistance may be accomplished by adjustment of the endothelial dysfunction as well as the skewed balance of adipokinesscience:S0065128116301234
  • Changes in Serum Nampt Levels and Its Significances in Diabetic Nephropathy Patients-The Potential Role of Nampt in T2DM with Diabetic Nephropathy.pubmed:28699485
  • Post-chemotherapy serum anti-müllerian hormone level predicts ovarian function recoveryPubmed:30012648
  • Effect of maternal hypothyroidism during pregnancy on insulin resistance, lipid accumulation and mitochondrial dysfunction in skeletal muscle of fetal ratsPubmed:29784871
  • Hyperinsulinemic-euglycemic clamp over the first year of use of depot-medroxyprogesterone acetate as a contraceptivePubmed:29665358
  • Analysis of synovial fluid visfatin level in temporomandibular joint disordersPubmed:29385907
  • Resistin and visfatin concentrations are related to central obesity and inflammation in Brazilian children10.1186:s41110-018-0060-7
  • Transcriptional Regulation of NAMPT Gene by Glycogen Synthase Kinase 3β in Goat AdipocytesPubmed: 30615481
  • The Effect of 8 Weeks Aquatic Exercise Program on Plasma Visfatin Level and Some Blood Factors in Obese Men
  • Adipokines as Biomarkers in Health and Disease
  • Plasma concentration and expression of adipokines in epicardial and subcutaneous adipose tissue are associated with impaired left ventricular filling patternPubmed: 31533725
  • Relationshıp of spontaneous subarachnoid haemorrhage and cerebral aneurysm to serum Visfatin and Nesfatin-1 levelsPubmed: 32311618
  • Effects of 10-Week Concurrent Training on Insulin Resistance and the Serum Levels of Vaspin and Visfatin in Overweight Females
  • Different Ratios of Corn and Coconut Oil Blends in High©\Fat Diets Influence Fat Deposition without Altering Metabolic Biomarkers in Male Rats

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