• KO-Validated
Monoclonal Antibody to Annexin A3 (ANXA3) Homo sapiens (Human) Monoclonal antibody

ANX-A3; ANX3; PAP-III; 35-alpha calcimedin; Inositol 1,2-cyclic phosphate 2-phosphohydrolase; Lipocortin III; Placental anticoagulant protein III

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Overview
Properties
  • SourceMonoclonal antibody preparation, Host Mouse
  • Ig Isotype IgG2a Kappa, Clone Number 2#
  • PurificationProtein A + Protein G affinity chromatography
  • LabelNone
  • Immunogen RPE786Hu01-Recombinant Annexin A3 (ANXA3)
  • Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
  • TraitsLiquid, Concentration 1mg/ml
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  • Monoclonal Antibody to Annexin A3 (ANXA3) Packages (Simulation)
  • Monoclonal Antibody to Annexin A3 (ANXA3) Packages (Simulation)
  • MAE786Hu21.jpg Figure. Western Blot; Sample: Recombinant ANXA3, Human.
  • Monoclonal Antibody to Annexin A3 (ANXA3) Western Blot; Sample: Lane1: Human Lung lysate; Lane2: HepG2 cell lysate
    Primary Ab: 3µg/ml Mouse Anti-Human ANXA3 Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu19) Selected
  • Monoclonal Antibody to Annexin A3 (ANXA3) Knockout Varification:
    Lane 1: Wild-type HepG2 cell lysate;
    Lane 2: ANXA3 knockout HepG2 cell lysate;
    Predicted MW: 36kDa
    Observed MW: 36kDa
    Primary Ab: 3µg/ml Mouse Anti-Human ANXA3 Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu19)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a mouse monoclonal antibody raised against ANXA3. It has been selected for its ability to recognize ANXA3 in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.01-3µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Organism Species More: Mus musculus (Mouse), Rattus norvegicus (Rat)

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