Monoclonal Antibody to Isocitrate Dehydrogenase 1, Soluble (IDH1) Homo sapiens (Human) Monoclonal antibody

PICD; IDP; Oxalosuccinate decarboxylase; NADP(+)-specific ICDH; Cytosolic NADP-isocitrate dehydrogenase

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Overview
Properties
  • SourceMonoclonal antibody preparation, Host Mouse
  • Ig Isotype IgG2a kappa, Clone Number C7
  • PurificationProtein A + Protein G affinity chromatography
  • LabelNone
  • Immunogen RPH839Hu01-Recombinant Isocitrate Dehydrogenase 1, Soluble (IDH1)
  • Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
  • TraitsLiquid, Concentration 1mg/ml
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  • Monoclonal Antibody to Isocitrate Dehydrogenase 1, Soluble (IDH1) Packages (Simulation)
  • Monoclonal Antibody to Isocitrate Dehydrogenase 1, Soluble (IDH1) Packages (Simulation)
  • Monoclonal Antibody to Isocitrate Dehydrogenase 1, Soluble (IDH1) DAB staining on IHC-P; Sample: Human Prostate Tissue; Primary Ab: 30µg/ml Mouse Anti-Human IDH1 Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19) Secondary antibody only control: Used PBS instead of primary antibody , Second Ab: 2µg/mL HRP-Linked
  • Monoclonal Antibody to Isocitrate Dehydrogenase 1, Soluble (IDH1) Vector Red staining on IHC-P;
    Samples: Human Adrenal Tissue;
    Primary Ab: 10µg/ml Mouse Anti-Human IDH1 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Monoclonal Antibody
  • MAH839Hu24.jpg Figure. Western Blot; Sample: Recombinant IDH1, Human.
  • Monoclonal Antibody to Isocitrate Dehydrogenase 1, Soluble (IDH1) Western Blot; Sample: Lane1: Human Liver Tissue; Lane2: Porcine Liver Tissue
    Primary Ab: 5µg/ml Mouse Anti-Human IDH1 Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
    (Catalog: SAA544Mu19) Selected
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a mouse monoclonal antibody raised against IDH1. It has been selected for its ability to recognize IDH1 in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.01-5µg/mL;
Immunohistochemistry: 5-30µg/mL;
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Organism Species More: Mus musculus (Mouse), Rattus norvegicus (Rat), Sus scrofa; Porcine (Pig)

Giveaways

Citations

  • Identification of IDH1 as a Potential Diagnostic and Prognostic Biomarker for Non-small-cell Lung Cancer by Proteomic AnalysisMcponline: 008821
  • Identification of Isocitrate Dehydrogenase 1 as a Potential Diagnostic and Prognostic Biomarker for Non-small Cell Lung Cancer by Proteomic AnalysisPubmed: 22064513
  • Isocitrate dehydrogenase 1 is a novel plasma biomarker for the diagnosis of non-small cell lung cancerPubmed: 24046070
  • The effects of PGC-1α on the proliferation and energy metabolism of malignant endometrial cancer cellsPubMed: 25914546
  • Metformin sensitizes endometrial cancer cells to chemotherapy through IDH1-induced Nrf2 expression via an epigenetic mechanismPubmed:29921847
  • IDH 2 is a novel diagnostic and prognostic serum biomarker for non‐small‐cell lung cancerPubmed:29465809
  • Serological Biomarkers for Early Detection of Hepatocellular Carcinoma: A Focus on Autoantibodies against Tumor-Associated Antigens Encoded by Cancer Driver …Pubmed: 32443439
  • Proteome profiling of enzalutamide‐resistant cell lines and serum analysis identified ALCAM as marker of resistance in castration‐resistant prostate cancerPubmed:35689436

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