• KO-Validated
Polyclonal Antibody to Acetyl Coenzyme A Acetyltransferase 1 (ACAT1) Homo sapiens (Human) Polyclonal antibody

THIL; ACAT; T2; MAT; Acetoacetyl Coenzyme A Thiolase

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Overview
Properties
  • SourcePolyclonal antibody preparation, Host Rabbit
  • Ig Type IgG, Potency n/a
  • PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
  • LabelNone
  • Immunogen RPD664Hu01-Recombinant Acetyl Coenzyme A Acetyltransferase 1 (ACAT1)
  • Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
  • TraitsLiquid, Concentration 0.71mg/ml
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  • Polyclonal Antibody to Acetyl Coenzyme A Acetyltransferase 1 (ACAT1) Packages (Simulation)
  • Polyclonal Antibody to Acetyl Coenzyme A Acetyltransferase 1 (ACAT1) Packages (Simulation)
  • Polyclonal Antibody to Acetyl Coenzyme A Acetyltransferase 1 (ACAT1) DAB staining on IHC-P; Samples: Human Kidney Tissue; Primary Ab: 20μg/ml Rabbit Anti-Human ACAT1 Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Acetyl Coenzyme A Acetyltransferase 1 (ACAT1) DAB staining on IHC-P;
    Samples: Human Lymph node Tissue;
    Primary Ab: 20μg/ml Rabbit Anti-Human ACAT1 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Acetyl Coenzyme A Acetyltransferase 1 (ACAT1) DAB staining on IHC-P;
    Sample: Human Kidney Tissue
    Primary Ab: 20µg/ml Rabbit Anti-Human ACAT1 Antibody
    Control: Used PBS instead of primary antibody
    Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Acetyl Coenzyme A Acetyltransferase 1 (ACAT1) DAB staining on IHC-P;
    Sample: Human Lymph node Tissue
    Primary Ab: 20µg/ml Rabbit Anti-Human ACAT1 Antibody
    Control: Used PBS instead of primary antibody
    Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Acetyl Coenzyme A Acetyltransferase 1 (ACAT1) FITC staining on IF;
    Samples: Human HepG2 cell;
    Primary Ab: 20μg/ml Rabbit Anti-Human ACAT1 Antibody
    Second Ab: 1.5μg/ml FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb18)
  • Polyclonal Antibody to Acetyl Coenzyme A Acetyltransferase 1 (ACAT1) FITC staining on IF;
    Sample: HepG2 cell
    Primary Ab: 20µg/ml Rabbit Anti-Human ACAT1 Antibody
    Second Ab: 2µg/ml FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb11)
  • Polyclonal Antibody to Acetyl Coenzyme A Acetyltransferase 1 (ACAT1) Knockout Varification:
    Lane 1: Wild-type A549 cell lysate;
    Lane 2: ACAT1 knockout A549 cell lysate;
    Predicted MW: 17,45kd
    Observed MW: 44kd
    Primary Ab: 1µg/ml Rabbit Anti-Human ACAT1 Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Acetyl Coenzyme A Acetyltransferase 1 (ACAT1) Human HepG2 cell was fixed with 2% paraformaldehyde (10 min) , permeabilised with 0.1% BSA-Triton X-100,then stained with 20µg/ml rabbit Anti-human ACAT1 Polyclonal Antibody (Catalog PAD664Hu01, red histogram) or Isotype control antibody (Catalog IS067-Rb01, green histogram), followed by 1µg/ml FITC-conjugated Anti-rabbit IgG Secondary Antibody (Catalog SAA544Rb18).
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against ACAT1. It has been selected for its ability to recognize ACAT1 in immunohistochemical staining and western blotting.

Usage

Immunohistochemistry: 5-20µg/mL;
Immunofluorescence:5-20µg/mL;
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Organism Species More: Sus scrofa; Porcine (Pig)

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