Polyclonal Antibody to Actin Alpha 1, Cardiac Muscle (ACTC1) Homo sapiens (Human) Polyclonal antibody

ACTC; CMD1R; CMD1-R; ACT-a1

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Overview
Properties
  • SourcePolyclonal antibody preparation, Host Rabbit
  • Ig Type IgG, Potency n/a
  • PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
  • LabelNone
  • Immunogen RPB341Hu01-Recombinant Actin Alpha 1, Cardiac Muscle (ACTC1)
  • Buffer FormulationPBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
  • TraitsLiquid, Concentration 500µg/mL
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  • Polyclonal Antibody to Actin Alpha 1, Cardiac Muscle (ACTC1) Packages (Simulation)
  • Polyclonal Antibody to Actin Alpha 1, Cardiac Muscle (ACTC1) Packages (Simulation)
  • Polyclonal Antibody to Actin Alpha 1, Cardiac Muscle (ACTC1) DAB staining on IHC-P; Samples: Human Esophagus Tissue; Primary Ab: 20µg/ml Rabbit Anti-Human ACTC1
  • Polyclonal Antibody to Actin Alpha 1, Cardiac Muscle (ACTC1) DAB staining on IHC-P;
    Samples: Human Esophagus Tissue
  • PAB341Hu01.jpg WB Image
  • Polyclonal Antibody to Actin Alpha 1, Cardiac Muscle (ACTC1) Western Blot; Sample: Mouse Heart lysate;
    Primary Ab: 1µg/ml Rabbit Anti-Human ACTC1 Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Actin Alpha 1, Cardiac Muscle (ACTC1) Western Blot; Sample: Lane1: Rat Heart lysate; Lane2: Mouse Heart lysate; Lane3: Porcine Heart lysate; Lane4: Canine Heart lysate; Lane5: Cavia Heart lysate
    Primary Ab: 1.5µg/mL Rabbit Anti-Human ACTC1 Ab
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Actin Alpha 1, Cardiac Muscle (ACTC1) Human Hela cell was fixed with 2% paraformaldehyde (10 min) , permeabilised with 0.1% BSA-Triton X-100,then stained with 20µg/ml rabbit Anti-human ACTC1 Polyclonal Antibody (Catalog PAB341Hu01, red histogram) or Isotype control antibody (Catalog IS067-Rb01, green histogram), followed by 1µg/ml FITC-conjugated Anti-rabbit IgG Secondary Antibody (Catalog SAA544Rb18).
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against ACTC1. It has been selected for its ability to recognize ACTC1 in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Organism Species More: Mus musculus (Mouse), Rattus norvegicus (Rat), Cavia (Guinea pig ), Canis familiaris; Canine (Dog), Sus scrofa; Porcine (Pig), Bos taurus; Bovine (Cattle), Equus caballus; Equine (Horse), Chicken (Gallus)

Giveaways

Citations

  • Plasma α-Actin as an Early Marker of Muscle Damage After Repeated Bouts of Eccentric CyclingPubmed:35522171

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