Polyclonal Antibody to GLI Family Zinc Finger Protein 3 (GLI3) Mus musculus (Mouse) Polyclonal antibody

GCPS, PHS; PAP-A; PAPA; PAPA1; PAPB; ACLS; PPDIV; Greig Cephalopolysyndactyly Syndrome; Glioma-Associated Oncogene Family Zinc Finger 3; GLI3 form of 190 kDa

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Overview
Properties
  • SourcePolyclonal antibody preparation, Host Rabbit
  • Ig Type IgG, Potency n/a
  • PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
  • LabelNone
  • Immunogen RPJ716Mu01-Recombinant GLI Family Zinc Finger Protein 3 (GLI3)
  • Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
  • TraitsLiquid, Concentration 500µg/mL
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  • Polyclonal Antibody to GLI Family Zinc Finger Protein 3 (GLI3) Packages (Simulation)
  • Polyclonal Antibody to GLI Family Zinc Finger Protein 3 (GLI3) Packages (Simulation)
  • Polyclonal Antibody to GLI Family Zinc Finger Protein 3 (GLI3) DAB staining on IHC-P; Samples: Mouse Colon Tissue; Primary Ab: 10µg/ml Rabbit Anti-Mouse GLI3 Anti
  • Polyclonal Antibody to GLI Family Zinc Finger Protein 3 (GLI3) DAB staining on IHC-P;
    Samples: Mouse Vas Deferens Tissue;
    Primary Ab: 10µg/ml Rabbit Anti-Mouse GLI3 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • PAJ716Mu01.jpg Figure. Western Blot; Sample: Recombinant GLI3, Mouse.
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against GLI3. It has been selected for its ability to recognize GLI3 in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.5-2µg/mL
Immunocytochemistry in formalin fixed cells: 5-20µg/mL
Immunohistochemistry in formalin fixed frozen section: 5-20µg/mL
Immunohistochemistry in paraffin section: 5-20µg/mL
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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