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ITEM PROTEIN ANTIBODY ASSAY KIT CELL
Polyclonal Antibody to Growth Differentiation Factor 6 (GDF6)
BMP13; CDMP2; GDF16
Organism species Mus musculus (Mouse)
Product No.PAC111Mu01
ClonalityPolyclonal
HostRabbit
Immunoglobulin TypeIgG
PurificationAffinity Chromatography
ApplicationsWB, ICC, IHC-P, IHC-F, ELISA
Concentration200ug/ml
UOM100ug
ConjugateNo Conjugate
IMMUNOGEN INFORMATION
Immunogen: Recombinant GDF6 (Ser339~Arg454) expressed in E.coli.
Accession No.: RPC111Mu01
Sequence: The target protein is fused with N-terminal His-Tag and its sequence is listed below.
MGHHHHHHSG SEF-SR HGKRHGKKSR LRCSRKPLHV NFKELGWDDW IIAPLEYEAY HCEGVCDFPL RSHLEPTNHA IIQTLMNSMD PGSTPPSCCV PTKLTPISIL YIDAGNNVVY KQYEDMVVES CGCR
ANTIBODY SPECIFITY
The antibody is a rabbit polyclonal antibody raised against GDF6. It has been selected for its ability to recognize GDF6 in immunohistochemical staining and western blotting.
APPLICATIONS
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.
CONTENTS
Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
QUALITY CONTROL
Content: The quality control contains recombinant GDF6 (Ser339~Arg454) disposed in loading buffer.
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
                5uL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control. Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol, BPB 0.01%, NaN3 0.02%.
STORAGE
Store at 4oC for frequent use. Stored at -20oC to -80oC in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Package and Components
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Benchmark price
Catalog No. Related products for research use of Mus musculus (Mouse) Organism species Applications
PAC111Mu01Polyclonal Antibody to Growth Differentiation Factor 6 (GDF6)WB, ICC, IHC-P, IHC-F, ELISA
RPC111Mu01Recombinant Growth Differentiation Factor 6 (GDF6)SDS-PAGE; WB; ELISA; IP.