Polyclonal Antibody to Kelch Like ECH Associated Protein 1 (KEAP1) Mus musculus (Mouse) Polyclonal antibody

INrf2; KLHL19; Cytosolic inhibitor of Nrf2; Kelch-like protein 19

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Overview
Properties
  • SourcePolyclonal antibody preparation, Host Rabbit
  • Ig Type IgG, Potency n/a
  • PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
  • LabelNone
  • Immunogen RPL648Mu01-Recombinant Kelch Like ECH Associated Protein 1 (KEAP1)
  • Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
  • TraitsLiquid, Concentration 0.5mg/ml
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  • Polyclonal Antibody to Kelch Like ECH Associated Protein 1 (KEAP1) Packages (Simulation)
  • Polyclonal Antibody to Kelch Like ECH Associated Protein 1 (KEAP1) Packages (Simulation)
  • Polyclonal Antibody to Kelch Like ECH Associated Protein 1 (KEAP1) IHC Images
  • Polyclonal Antibody to Kelch Like ECH Associated Protein 1 (KEAP1) AF488 staining on IF;
    Sample: HepG2 cell
    Primary Ab: 20µg/ml Rabbit Anti-Mouse KEAP1 Antibody
    Second Ab: 2μg/ml AF488-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb11)
  • PAL648Mu01.jpg Figure. Western Blot; Sample: Recombinant KEAP1, Mouse.
  • Polyclonal Antibody to Kelch Like ECH Associated Protein 1 (KEAP1) Western Blot; Sample: Mouse Serum;
    Primary Ab: 1µg/ml Rabbit Anti-Mouse KEAP1 Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Kelch Like ECH Associated Protein 1 (KEAP1) Western Blot; Samples: Lane1: NIH/3T3 cell lysate; Lane2: RAW264.7 cell lysate;
    Primary Ab: 0.2µg/ml Rabbit Anti-Mouse KEAP1 Antibody
    Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against KEAP1. It has been selected for its ability to recognize KEAP1 in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.01-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Organism Species More: Homo sapiens (Human), Rattus norvegicus (Rat)

Giveaways

Citations

  • Ameliorative potential of linagliptin and/or calcipotriol on bleomycin-induced lung fibrosis: In vivo and in vitro studypubmed:28192751
  • Targeting the proinflammatory cytokines, oxidative stress, apoptosis and TGF-β1/STAT-3 signaling by irbesartan to ameliorate doxorubicin-induced hepatotoxicityPubmed:29753615
  • Protective effect of sesamin in lipopolysaccharide-induced mouse model of acute kidney injury via attenuation of oxidative stress, inflammation, and apoptosisPubmed: 30488751
  • S-allyl cysteine protects against lipopolysaccharide-induced acute kidney injury in the C57BL/6 mouse strain: Involvement of oxidative stress and inflammationPubmed: 30665040
  • Targeting oxidative stress, proinflammatory cytokines, apoptosis and toll like receptor 4 by empagliflozin to ameliorate bleomycin-induced lung fibrosisPubmed: 31600583
  • Protective and Therapeutic Effects of Orlistat on Metabolic Syndrome and Oxidative Stress in High-Fat Diet-Induced Metabolic Dysfunction-Associated Fatty Liver …34822647
  • Hepatoprotective Effect of Bee Bread in Metabolic Dysfunction-Associated Fatty Liver Disease (MAFLD) Rats: Impact on Oxidative Stress and Inflammation34943134

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