Home  |   About us
ITEM PROTEIN ANTIBODY ASSAY KIT CELL
Polyclonal Antibody to Peripheral Myelin Protein 22 (PMP22)
DSS; CMT1A; CMT1E; GAS3; HMSNIA; HNPP; Sp110
Organism species Homo sapiens (Human)
Product No.PAC746Hu01
ClonalityPolyclonal
HostRabbit
Immunoglobulin TypeIgG
PurificationAffinity Chromatography
ApplicationsWB, ICC, IHC-P, IHC-F, ELISA
Concentration200ug/ml
UOM100ug
ConjugateNo Conjugate
IMMUNOGEN INFORMATION
Immunogen: Recombinant PMP22 (Met1~Glu160) expressed in E.coli.
Accession No.: RPC746Hu01
Sequence: The target protein is fused with two N-terminal Tags, His-tag and its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF-MLLLLLSIIV LHVAVLVLLF VSTIVSQWIV GNGHATDLWQ NCSTSSSGNV HHCFSSSPNE WLQSVQATMI LSIIFSILSL FLFFCQLFTL TKGGRFYITG IFQILAGLCV MSAAAIYTVR HPEWHLNSDY SYGFAYILAW VAFPLALLSG VIYVILRKRE
ANTIBODY SPECIFITY
The antibody is a rabbit polyclonal antibody raised against PMP22. It has been selected for its ability to recognize PMP22 in immunohistochemical staining and western blotting.
APPLICATIONS
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.
CONTENTS
Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
QUALITY CONTROL
Content: The quality control contains recombinant Thyroglobulin (Ser2083~Ala2333) disposed in loading buffer.
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
                5uL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control. Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol, BPB 0.01%, NaN3 0.02%.
STORAGE
Store at 4oC for frequent use. Stored at -20oC to -80oC in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Package and Components
Download
Benchmark price
Catalog No. Related products for research use of Homo sapiens (Human) Organism species Applications
MAC746Hu21Monoclonal Antibody to Peripheral Myelin Protein 22 (PMP22)WB, ICC, IHC-P, IHC-F, ELISA
PAC746Hu01Polyclonal Antibody to Peripheral Myelin Protein 22 (PMP22)WB, ICC, IHC-P, IHC-F, ELISA
RPC746Hu01Recombinant Peripheral Myelin Protein 22 (PMP22)SDS-PAGE; WB; ELISA; IP.
SEC746HuELISA Kit for Peripheral Myelin Protein 22 (PMP22)Enzyme-linked immunosorbent assay