CLIA Kit for Amyloid Precursor Protein (APP) Homo sapiens (Human) Sandwich CLIA

AAA; ABeta; ABPP; AD1; APPI; CVAP; PN2; CTFgamma; Peptidase Nexin-II Alzheimer Disease; Peptidase Nexin-II; Amyloid Beta(A4)Precursor Protein

  • Product No.SCB020Hu
  • Organism SpeciesHomo sapiens (Human)
  • Sample TypeSerum, plasma, cerebrospinal fluid and other biological fluids
  • Test MethodDouble-antibody Sandwich
  • Assay Length2h, 40min
  • Detection Range78.12-5000pg/mL
  • SensitivityThe minimum detectable dose of this kit is typically less than 2.43pg/mL.
  • Specificity This assay has high sensitivity and excellent specificity for detection of Amyloid Precursor Protein (APP).
    No significant cross-reactivity or interference between Amyloid Precursor Protein (APP) and analogues was observed.
  • ApplicationsChemiluminescent immunoassay for Antigen Detection
  • DownloadInstruction Manual
  • Format 48T96T 96T*5 96T*10 96T*100
  • FOB US$ 588US$ 840 US$ 3780 US$ 7140 US$ 58800
  • CLIA Kit for Amyloid Precursor Protein (APP) Package and Components
  • CLIA Kit for Amyloid Precursor Protein (APP) Package and Components
  • CLIA Kit for Amyloid Precursor Protein (APP) Results demonstration
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Recovery

Matrices listed below were spiked with certain level of recombinant Amyloid Precursor Protein (APP) and the recovery rates were calculated by comparing the measured value to the expected amount of Amyloid Precursor Protein (APP) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 90-102 98
EDTA plasma(n=5) 92-101 96
heparin plasma(n=5) 79-90 83

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Amyloid Precursor Protein (APP) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Amyloid Precursor Protein (APP) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Amyloid Precursor Protein (APP) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 97-105% 82-93% 93-101% 97-105%
EDTA plasma(n=5) 98-105% 81-102% 97-105% 92-105%
heparin plasma(n=5) 79-101% 94-102% 93-104% 84-101%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
Substrate A 1×10mL Substrate B 1×2mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 100µL Substrate Solution. Incubate 10 minutes at 37°C;
8. Read RLU value immediately.

CLIA Kit for Amyloid Precursor Protein (APP)

Test principle

The microtiter plate provided in this kit has been pre-coated with an antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated secondary antibody. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Amyloid Precursor Protein (APP) level in the sample or standard.

GIVEAWAYS

Reference

  • Neurochemistry InternationalBilobalide regulates soluble amyloid precursor protein release via phosphatidyl inositol 3 kinase-dependent pathwayScienceDirect: S0197018611001641
  • NeuroscienceSubstitution of membrane cholesterol with β-sitosterol promotes nonamyloidogenic cleavage of endogenous amyloid precursor proteinPubmed: 23707801