CLIA Kit for Angiotensin I Converting Enzyme (ACE)Homo sapiens (Human)Sandwich CLIA

CD143; ACE1; DCP1; ACEI; ACE-I; Kininase II; Angiotensin-Converting Enzyme; Peptidyl-Dipeptidase A; Dipeptidyl Carboxypeptidase 1; Angiotensin-converting enzyme, soluble form

  • Product No.SCA004Hu
  • Organism SpeciesHomo sapiens (Human)Same name, Different species.
  • Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
  • Test MethodDouble-antibody Sandwich
  • Assay Length2h, 40min
  • Detection Range68.6-50,000pg/mL
  • SensitivityThe minimum detectable dose of this kit is typically less than 24.2pg/mL.
  • Specificity This assay has high sensitivity and excellent specificity for detection of Angiotensin I Converting Enzyme (ACE).
    No significant cross-reactivity or interference between Angiotensin I Converting Enzyme (ACE) and analogues was observed.
  • ApplicationsChemiluminescent immunoassay for Antigen Detection
  • DownloadInstruction Manual
  • Format48T96T96T*596T*1096T*100
  • FOBUS$ 588US$ 840US$ 3780US$ 7140US$ 58800
  • CLIA Kit for Angiotensin I Converting Enzyme (ACE)Package and Components
  • CLIA Kit for Angiotensin I Converting Enzyme (ACE)Package and Components
  • CLIA Kit for Angiotensin I Converting Enzyme (ACE)Results demonstration
  • SCA004Hu.jpgTypical Standard Curve
  • CertificateISO9001: 2008, ISO13485: 2003 Registered

Recovery

Matrices listed below were spiked with certain level of recombinant Angiotensin I Converting Enzyme (ACE) and the recovery rates were calculated by comparing the measured value to the expected amount of Angiotensin I Converting Enzyme (ACE) in samples.

MatrixRecovery range (%)Average(%)
serum(n=5)89-9692
EDTA plasma(n=5)83-10192
heparin plasma(n=5)82-9895

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Angiotensin I Converting Enzyme (ACE) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Angiotensin I Converting Enzyme (ACE) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Angiotensin I Converting Enzyme (ACE) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample1:21:41:81:16
serum(n=5)90-98%81-97%89-103%89-103%
EDTA plasma(n=5)95-105%94-102%78-99%80-88%
heparin plasma(n=5)80-105%85-99%89-98%89-98%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

ReagentsQuantityReagentsQuantity
Pre-coated, ready to use 96-well strip plate1Plate sealer for 96 wells4
Standard2Standard Diluent1×20mL
Detection Reagent A1×120µLAssay Diluent A1×12mL
Detection Reagent B1×120µLAssay Diluent B1×12mL
Substrate A1×10mLSubstrate B1×2mL
Wash Buffer (30 × concentrate)1×20mLInstruction manual1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 100µL Substrate Solution. Incubate 10 minutes at 37°C;
8. Read RLU value immediately.

CLIA Kit for Angiotensin I Converting Enzyme (ACE)

Test principle

The microtiter plate provided in this kit has been pre-coated with an antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated secondary antibody. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Angiotensin I Converting Enzyme (ACE) level in the sample or standard.

Reference

  • Lipids in Health and diseaseAssociation of elevated blood pressure and impaired vasorelaxation in experimental Sprague-Dawley rats fed with heated vegetable oilPubMed: PMC2914008
  • Malaysian Journal of Medical SciencesEffect of Repeatedly Heated Palm Olein on Blood Pressureā€“Regulating Enzymes Activity and Lipid Peroxidation in RatsUsm: MJMS19120
  • Journal of Cancer TherapyPlasma Levels of Angiotensin-Converting Enzymes 1 and 2 and AGTR2 (T1247G and A5235G) Gene Polymorphisms Are Associated to Breast Cancer Progression.Scirp: Source
  • The American journal of clinical nutritionVascular function and atherosclerosis progression after 1 y of flavonoid intake in statin-treated postmenopausal women with type 2 diabetes: a double-blind randomized controlled trial.Pubmed: 23553151
  • Journal of Vascular SurgeryCathepsin G deficiency reduces periaortic calcium chloride injury-induced abdominal aortic aneurysms in mice Pubmed:25037606
  • Reprod Toxicol.?ACE2 activation by xanthenone prevents leptin-induced increases in blood pressure and proteinuria during pregnancy in Sprague-Dawley ratsPubmed:25205467
  • Chemical Research in ToxicologySub-chronic Toxicity and Cardiovascular Responses in Spontaneously Hypertensive Rats after Exposure to Multiwall Carbon Nanotubes by Intratracheal InstillationPubmed:25580880
  • Chem Biol InteractArsenic causes aortic dysfunction and systemic hypertension in rats: Augmentation of angiotensin II signalingPubMed: 26079204
  • Biomed Res IntProtective Effect of Enalapril against Methionine-Enriched Diet-Induced Hypertension: Role of Endoplasmic Reticulum and Oxidative StressPubMed: 26640794
  • Clinics (Sao Paulo)MechanismsPubMed: 26602523
  • Chemical Research in ToxicologySubchronic Toxicity and Cardiovascular Responses in Spontaneously Hypertensive Rats after Exposure to Multiwalled Carbon Nanotubes by Intratracheal InstillationPubMed: 25580880
  • Intensive Care Medicine Experimental Angiotensin converting enzymes in patients with acute respiratory distress syndromeContent: 3