CLIA Kit for Angiotensinogen (AGT)Homo sapiens (Human)Sandwich CLIA

ANHU; SERPINA8; Angiotensin; Renin Substrate; Serpin Peptidase Inhibitor,Clade A,Member 8(Alpha-1 Antiproteinase,Antitrypsin)

  • Product No.SCA797Hu
  • Organism SpeciesHomo sapiens (Human)Same name, Different species.
  • Sample TypeSerum, plasma and other biological fluids
  • Test MethodDouble-antibody Sandwich
  • Assay Length2h, 40min
  • Detection Range1.56-100ng/mL
  • SensitivityThe minimum detectable dose of this kit is typically less than 0.07ng/mL.
  • Specificity This assay has high sensitivity and excellent specificity for detection of Angiotensinogen (AGT).
    No significant cross-reactivity or interference between Angiotensinogen (AGT) and analogues was observed.
  • ApplicationsChemiluminescent immunoassay for Antigen Detection
  • DownloadInstruction Manual
  • Format48T96T96T*596T*1096T*100
  • FOBUS$ 588US$ 840US$ 3780US$ 7140US$ 58800
  • CLIA Kit for Angiotensinogen (AGT)Package and Components
  • CLIA Kit for Angiotensinogen (AGT)Package and Components
  • CLIA Kit for Angiotensinogen (AGT)Results demonstration
  • CertificateISO9001: 2008, ISO13485: 2003 Registered

Recovery

Matrices listed below were spiked with certain level of recombinant Angiotensinogen (AGT) and the recovery rates were calculated by comparing the measured value to the expected amount of Angiotensinogen (AGT) in samples.

MatrixRecovery range (%)Average(%)
serum(n=5)83-9790
EDTA plasma(n=5)84-9890
heparin plasma(n=5)98-105102

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Angiotensinogen (AGT) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Angiotensinogen (AGT) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Angiotensinogen (AGT) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample1:21:41:81:16
serum(n=5)82-103%88-98%96-105%94-104%
EDTA plasma(n=5)92-103%97-105%95-102%89-98%
heparin plasma(n=5)80-97%91-98%99-105%84-105%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

ReagentsQuantityReagentsQuantity
Pre-coated, ready to use 96-well strip plate1Plate sealer for 96 wells4
Standard2Standard Diluent1×20mL
Detection Reagent A1×120µLAssay Diluent A1×12mL
Detection Reagent B1×120µLAssay Diluent B1×12mL
Substrate A1×10mLSubstrate B1×2mL
Wash Buffer (30 × concentrate)1×20mLInstruction manual1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 100µL Substrate Solution. Incubate 10 minutes at 37°C;
8. Read RLU value immediately.

CLIA Kit for Angiotensinogen (AGT)

Test principle

The microtiter plate provided in this kit has been pre-coated with an antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated secondary antibody. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Angiotensinogen (AGT) level in the sample or standard.

Reference

  • Transplant InternationalElevated urinary angiotensinogen a marker of intrarenal renin angiotensin system in hypertensive renal transplant recipients: does it play a role in development of proteinuria in hypertensive renal transplant patients?Wiley: source
  • AmyloidThe relation between urinary angiotensinogen and proteinuria in renal AA amyloidosis patientsPubMed: 22320202
  • Pediatric MephrologyUrinary angiotensinogen as a marker of intrarenal angiotensin II activity in adolescents with primary hypertensionPubMed: PMC3661928
  • Acta PaediatricaUrinary angiotensinogen as a novel marker of obstructive nephropathy in childrenPubmed: 23772991
  • Journal of the Renin-Angiotensin-Aldosterone SystemAssociation between urinary angiotensinogen, hypertension and proteinuria in pregnant women with preeclampsiaSagepub: Source
  • Pediatric NephrologyMP707 CHARACTERISTICS AND OUTCOMES OF CHILDREN WITH AUTOSOMAL RECESSIVE POLYCYSTIC KIDNEY DISEASE COHORT FROM THE ESPN/ERA-EDTA REGISTRYOxfordjournals:Source
  • journal of human hypertensionRelationship between blood pressure varPubMed: 26223347
  • PLoS OneHigher Serum Angiotensinogen Is an Indicator of IgA Vasculitis with Nephritis Revealed by Comparative Proteomes AnalysisPubMed: 26098644
  • MKAKorelasi pengukuran antropometri dengan tekanan darah dan angiotensinogen plasma pada dewasaIndex.Php: Art