CLIA Kit for Brain Derived Neurotrophic Factor (BDNF) Homo sapiens (Human) Sandwich CLIA

Neurotrophin; Abrineurin

  • CLIA Kit for Brain Derived Neurotrophic Factor (BDNF) Packages (Simulation)
  • CLIA Kit for Brain Derived Neurotrophic Factor (BDNF) Packages (Simulation)
  • CLIA Kit for Brain Derived Neurotrophic Factor (BDNF) Results demonstration
  • SCA011Hu.jpg Typical Standard Curve
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Recovery

Matrices listed below were spiked with certain level of recombinant Brain Derived Neurotrophic Factor (BDNF) and the recovery rates were calculated by comparing the measured value to the expected amount of Brain Derived Neurotrophic Factor (BDNF) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 81-91 87
EDTA plasma(n=5) 85-101 90
heparin plasma(n=5) 92-101 96

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Brain Derived Neurotrophic Factor (BDNF) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Brain Derived Neurotrophic Factor (BDNF) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Brain Derived Neurotrophic Factor (BDNF) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 94-102% 99-105% 93-105% 91-103%
EDTA plasma(n=5) 89-97% 83-95% 93-105% 80-97%
heparin plasma(n=5) 91-104% 86-94% 79-104% 98-105%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
Substrate A 1×10mL Substrate B 1×2mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 100µL Substrate Solution. Incubate 10 minutes at 37°C;
8. Read RLU value immediately.

CLIA Kit for Brain Derived Neurotrophic Factor (BDNF)

Test principle

The microplate provided in this kit has been pre-coated with an antibody specific to Brain Derived Neurotrophic Factor (BDNF). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Brain Derived Neurotrophic Factor (BDNF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Brain Derived Neurotrophic Factor (BDNF) level in the sample or standard.;

Reference

  • Toxicological SciencesInteraction of PFOS and BDE-47 Co-exposure on Thyroid Hormone Levels and TH-Related Gene and Protein Expression in Developing Rat BrainsPubMed: 21436126
  • Journal of the Neurological SciencesNeuroprotective effects of adipose-derived stem cells against ischemic neuronal damage in the rabbit spinal cordScienceDirect: S0022510X12001219
  • International Conference on BioscienceEffect of Benthosemapterotum Peptide on Aging in Mice with Oxidative DamagePsrcentre: 212059
  • Neuroscience LettersChronic stress impairs learning and hippocampal cell proliferation in senescence-accelerated prone miceScienceDirect: S0304394010015788
  • BioMed Research InternationalNeuroprotective effects of adipose-derived stem cells are maintained for 3 weeks against ischemic damage in the rabbit spinal cord.NCBI: PMC3925622
  • Behavioural Brain ResearchThe effects of Ginkgo biloba extract on cognitive functions in aged female rats: The role of oxidative stress and brain-derived neurotrophic factorPubmed:25446810
  • Neurochemical ResearchHeme Oxygenase-1 Protects Neurons from Ischemic Damage by UpreCavia (Guinea pig )lating Expression of Cu,Zn-SuperoxideDismutase, Catalase, and Brain-Derived Neurotrophic Factor in the Rabbit Spinal Cord.Pubmed:26559686
  • Exp Ther Med. Effects of docosahexaenoic acid on learning and memory impairment induced by repeated propofol anesthesia in young ratspmc:PMC4812310
  • Genet Mol ResChanges in growth factor levels in the cerebrospinal fluid of autism patients after transplantation of human umbilical cord blood mononuclear cells and umbilical cord-derived mesenchymal stem cellsPubmed:27323064
  • The International Journal af Applied Research in Veterinary MedicineEffect of Sample Time in Clot Tube, Temperature, and Hemolysis Status on Canine Serum Brain-Derived Neurotrophic Factor (BDNF) Concentrations: Preliminary study articles:Vol14Iss3
  • J Chem Neuroanat.Both secreted and the cellular levels of BDNF attenuated due to tau hyperphosphorylation in primary cultures of cortical neurons.pubmed:27914953
  • Int Immunopharmacol.PPARr activation ameliorates postoperative cognitive decline probably through suppressing hippocampal neuroinflammation in aged mice.pubmed:27940378
  • The Journal of Maternal-Fetal & Neonatal MedicineDifferential expression of cord blood neurotrophins in gestational diabetes: the impact of fetal growth abnormalitiespubmed:28081639
  • Bipolar DisordersLong‐term lithium treatment increases intracellular and extracellular brain‐derived neurotrophic factor (BDNF) in cortical and hippocampal neurons at subtherapeutic …bdi.12449
  • ElsevierAssociations of blood parameters with age, feed efficiency and sampling routine in young beef bullsscience:article