CLIA Kit for Erythropoietin (EPO) Homo sapiens (Human) Sandwich CLIA

EP; Epoetin; Erythropoetin; Hematopoietin; Hemopoietin

  • CLIA Kit for Erythropoietin (EPO) Packages (Simulation)
  • CLIA Kit for Erythropoietin (EPO) Packages (Simulation)
  • CLIA Kit for Erythropoietin (EPO) Results demonstration
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Erythropoietin (EPO) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Erythropoietin (EPO) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
Substrate A 1×10mL Substrate B 1×2mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 100µL Substrate Solution. Incubate 10 minutes at 37°C;
8. Read RLU value immediately.

CLIA Kit for Erythropoietin (EPO)

Test principle

The microplate provided in this kit has been pre-coated with an antibody specific to Erythropoietin (EPO). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Erythropoietin (EPO). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Erythropoietin (EPO) level in the sample or standard.;

Reference

  • EndocrinologyPrenatal Glucocorticoid Overexposure Causes Permanent Increases in Renal Erythropoietin Expression and Red Blood Cell Mass in the Rat OffspringEndo: source
  • Journal of EthnopharmacologyInhibitory effect of polysaccharides isolated from Angelica sinensis on hepcidin expressionPubMed: 21333724
  • PLOS ONECD98 Positive Eosinophils Contribute to T Helper 1 Pattern InflammationPlosOne: Source
  • The Journal of Clinical Endocrinology & MetabolismAlterations of Circulating Endothelial Cell and Endothelial Progenitor Cell Counts around the OvulationPubMed: 22948762
  •  Experimental & Molecular MedicineLong-term and stable correction of uremic anemia by intramuscular injection of plasmids containing hypoxia-regulated system of erythropoietin expressionPubMed: PMC3509184
  • Biochem Biophys Res Commun.Reevaluation of erythropoietin production by the nephronPubmed:24832733
  • Nutrition. Carbohydrate and glutamine supplementation modulates the Th1/Th2 balance after exercise performed at a simulated altitude of 4500 m.Pubmed:2528040
  • J Appl Physiol (1985).Decreased plasma soluble erythropoietin receptor in high-altitude excessive erythrocytosis and Chronic Mountain SicknessPubmed:25324511
  • Expert Rev ProteomicsPlasma hepcidin in early-stage breast cancer patients: no relationship with interleukin-6, erythropoietin and erythroferronePubMed: 26496240