CLIA Kit for Interleukin 6 (IL6)Homo sapiens (Human)Sandwich CLIA

MGI2-A; MGI2A; HGF; BSF2; HSF; IFNB2; B-Cell Stimulatory Factor-2; Hybridoma/Plasmacytoma Growth Factor; Hepatocyte Stimulating Factor; Cytotoxic T-Cell Differentiation Factor

  • Product No.SCA079Hu
  • Organism SpeciesHomo sapiens (Human)Same name, Different species.
  • Sample TypeSerum, plasma, tissue homogenates, cell culture supernates and other biological fluids
  • Test MethodDouble-antibody Sandwich
  • Assay Length2h, 40min
  • Detection Range31.25-2000pg/mL
  • SensitivityThe minimum detectable dose of this kit is typically less than 1.18pg/mL.
  • Specificity This assay has high sensitivity and excellent specificity for detection of Interleukin 6 (IL6).
    No significant cross-reactivity or interference between Interleukin 6 (IL6) and analogues was observed.
  • ApplicationsChemiluminescent immunoassay for Antigen Detection
  • DownloadInstruction Manual
  • Format48T96T96T*596T*1096T*100
  • FOBUS$ 588US$ 840US$ 3780US$ 7140US$ 58800
  • CLIA Kit for Interleukin 6 (IL6)Package and Components
  • CLIA Kit for Interleukin 6 (IL6)Package and Components
  • CLIA Kit for Interleukin 6 (IL6)Results demonstration
  • CertificateISO9001: 2008, ISO13485: 2003 Registered

Recovery

Matrices listed below were spiked with certain level of recombinant Interleukin 6 (IL6) and the recovery rates were calculated by comparing the measured value to the expected amount of Interleukin 6 (IL6) in samples.

MatrixRecovery range (%)Average(%)
serum(n=5)97-105101
EDTA plasma(n=5)79-8879
heparin plasma(n=5)96-104101

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interleukin 6 (IL6) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interleukin 6 (IL6) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Interleukin 6 (IL6) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample1:21:41:81:16
serum(n=5)98-105%96-104%89-104%99-105%
EDTA plasma(n=5)94-101%79-103%98-105%95-104%
heparin plasma(n=5)79-95%85-99%78-95%92-99%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

ReagentsQuantityReagentsQuantity
Pre-coated, ready to use 96-well strip plate1Plate sealer for 96 wells4
Standard2Standard Diluent1×20mL
Detection Reagent A1×120µLAssay Diluent A1×12mL
Detection Reagent B1×120µLAssay Diluent B1×12mL
Substrate A1×10mLSubstrate B1×2mL
Wash Buffer (30 × concentrate)1×20mLInstruction manual1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 100µL Substrate Solution. Incubate 10 minutes at 37°C;
8. Read RLU value immediately.

CLIA Kit for Interleukin 6 (IL6)

Test principle

The microtiter plate provided in this kit has been pre-coated with an antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated secondary antibody. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Interleukin 6 (IL6) level in the sample or standard.

Reference

  • Journal of Korean Neurosurgical SocietyNotochordal Cells Influence Gene Expression of Inflammatory Mediators of Annulus Fibrosus Cells in Proinflammatory Cytokines StimulationPubMed: PMC2916142
  • Experimental AnimalsAnti-Inflammatory Effect of Guan-Xin-Er-Hao via the Nuclear Factor-Kappa B Signaling Pathway in Rats with Acute Myocardial InfarctionJstage: source
  • Journal of Translational MedicineLow temperature of radiofrequency ablation at the target sites can facilitate rapid progression of residual hepatic VX2 carcinomaBioMed: 14795876
  • IUBMB LIFE Genetic repression of mouse VEGF expression regulates coagulation cascade.PubMed: 21086498
  • Not availableAnalysis of genetic markers for cardiovascular disorders in a portuguese population with familial hypercholesterolemiaUniversi: 49434630
  • Acta OtolaryngolSerial cytokine levels during wound healing in rabbit maxillary sinus mucosaPubMed: 19958244
  • The EMBO Journal Egr-1 decreases adipocyte insulin sensitivity by tilting PI3K/Akt and MAPK signal balance in miceNature: 2011277
  • Reproductive BioMedicine OnlineSoluble BAFF-R produced by decidual stromal cells plays an inhibitory role in monocytes and macrophages ScienceDirect: S1472648312001484
  • CarcinogenesisThe association of interleukin-16 polymorphisms with IL-16 serum levels and risk of colorectal and gastric cancerOxfordJournals: source
  • Diabetes Research and Clinical PracticePlasma SFRP5 levels are decreased in Chinese subjects with obesity and type 2 diabetes and negatively correlated with parameters of insulin resistancePubMed: 23290274
  • BoneSynthesis and inflammatory response of a novel silk fibroin scaffold containing BMP7 adenovirus for bone regenerationPubMed: 22796416
  • Heart and Education in HeartThe change of serum level of pigment epithelium-derived factor in coronary heart diseaseBmj: Source
  • Journal of Surgical ResearchEnhanced tumor growth after portal vein embolization in a rabbit tumor modelPubMed: 23149224
  • NanomedicineEfficient intracellular delivery of siRNA with a safe multitargeted lipid-based nanoplatformPubMed: 23394132
  • PLoS OneNew Method to Isolate and Culture Rat Kupffer CellsPubMed: PMC3743898
  • PLoS OneRecombinant Adiponectin Ameliorates Liver Ischemia Reperfusion Injury via Activating the AMPK/eNOS PathwayPubMed: PMC3676360
  • PLoS One.Molecular Effects of Doxycycline Treatment on Pterygium as Revealed by Massive Transcriptome SequencingPubMed: PMC3378547
  • Oncology Reports A bone-seeking clone exhibits different biological properties from the ACHN parental human renal cell carcinoma in vivo and in vitroPubMed: PMC3583590
  • Experimental and Therapeutic MedicineShuangtengbitong tincture treatment of collagen-induced arthritis via downregulation of the expression of IL-6, IL-8, TNF-α and NF-κBPubMed: PMC3570159
  • 6th International Scientific Interdisciplinary Conference (ISIC)Inflammation influence on the formation of endothelial dysfunction in hypertensive patients caused by obesityKnmu: Source
  • Lower Urinary Tract SymptomsEffects of Saw Palmetto Extract on Urodynamic Parameters, Bladder Muscarinic and Purinergic Receptors and Urinary Cytokines in Rats with Cyclophosphamide-Induced CystitisOnlinelibrary: luts.12015
  • Polish Journal of Veterinary SciencesThe effect of different dietary levels of vitamin E and selenium on antioxidant status and immunological markers in serum of laying hensPubmed: 23971202
  • Journal of natural remediesIxora coccinea down Regulate the Inflammation-Associated Prostaglandins (PGE-2), Nitric Oxide (NO) and Cytokines Production in LPS-Stimulated RAW 264.7 MacrophageJnronline: Source
  • The Veterinary JournalMonocyte chemotactic protein-1 and other inflammatory parameters in Bernese Mountain dogs with disseminated histiocytic sarcomaPubmed: 23992872
  • Archives of Medical ResearchEffect of α-Linolenic Acid on Streptozotocin-induced Diabetic Retinopathy Indices In VivoPubmed: 24120388
  • Glob J Health Sci. Inflammatory markers and haptoglobin polymorphism in Saudi with non-insulin-dependent diabetes mellitus.Pubmed: 23283045
  • Inflamm Res.Intracranial injection of recombinant stromal-derived factor-1 alpha (SDF-1α) attenuates traumatic brain injury in rats.Pubmed: 24352531
  • Molecules. Identification of compounds from the water soluble extract of Cinnamomum cassia barks and their inhibitory effects against high-glucose-induced mesangial cells.Pubmed: 24013407
  • PLoS One. Megakaryocytic leukemia 1 (MKL1) regulates hypoxia induced pulmonary hypertension in rats.Pubmed: 24647044
  • J Surg Res. Immunogenic alteration in laparoscopic common bile duct exploration.Pubmed: 24239148
  • BMC anesthesiologyRepeated open endotracheal suctioning causes gradual desaturation but does not exacerbate lung injury compared to closed endotracheal suctioning in a rabbit model of ARDS.Pubmed: 24308643
  • Physiological ResearchBudesonide added to modified porcine surfactant Curosurf may additionally improve the lung functions in meconium aspiration syndrome.Pubmed: 2432969
  • Journal of EthnopharmacologyBioactive compounds from Cornus officinalis fruits and their effects on diabetic nephropathyScienceDirect: S0378874114002414
  • PLoS One.HIF-1α Is Essential for Effective PMN Bacterial Killing, Antimicrobial Peptide Production and Apoptosis in Pseudomonas aeruginosa KeratitisPlos: Source
  • Journal of Materials Chemistry BNanocomposites of bio-based hyperbranched polyurethane/funtionalized MWCNT as non-immunogenic, osteoconductive, biodegradable and biocompatible scaffolds in bone tissue engineeringRsc: Source
  • Biosensors and BioelectronicsA new signal amplification strategy of photoelectrochemical immunoassay for highly sensitive interleukin-6 detection based on TiO2/CdS/CdSe dual co-sensitized structureScienceDirect: S0956566314001924
  •  Journal of Pharmaceutical Sciences and PharmacologyAnti-inflammatory Effect of Euphorbia hirta Leaf Extract in Rats and Modulation of Inflammation-Associated Prostaglandins (PGE-2) and Nitric Oxide (NO) Expression in RAW264.7 MacrophageIngentaconnect: Source
  • Veterinary ResearchImmunosuppression abrogates resistance of young rabbits to Rabbit Haemorrhagic Disease (RHD)Biomedcentral: Source
  • Biochem Biophys Res Commun.Dipeptidyl peptidase-4 inhibitor ameliorates early renal injury through its anti-inflammatory action in a rat model of type 1 diabetesPubmed: 24342619
  • INTERNATIONAL JOURNAL OF MEDICINE AND MEDICAL RESEARCHL-ARGININE, BUT NOT L-NAME PROTECTS AGAINST LIVER INJURY INDUCED BY EXPERIMENTAL ISCHEMIA-REPERFUSIONTdmu:Source
  • Anesthesia &amp; Clinical
ResearchEffect of High Pressure-Volume and Low Pressure-Volume Mechanical Ventilation on Plasma Concentrations of Inflammatory Markers in Horses during General …Org:Source
  • Basic &amp; Clinical Pharmacology &amp; ToxicologyTreatment with Carnitine Enhances Bone Fracture Healing under Osteoporotic and/or Inflammatory ConditionsWiley:Source
  • New Journal of ChemistryBiophysico-chemical interfacial attributes of Fe3O4 decorated MWCNT nanohybrid/bio-based hyperbranched polyurethane nanocomposite: an antibacterial wound healing material with controlled drug release potentialRsc:Source
  • 芦荟大黄素抑制LPS诱导RAW264.7细胞释放炎症因子作用机制研究Docin:Source
  • Evidence-Based Complementary and Alternative MedicineGinsenosides Rg1 from Panax ginseng: A Potential Therapy for Acute Liver Failure Patients?Pubmed:Pmc4241314
  • Pharmacognosy Res.Margaritaria discoidea (Euphorbiaceae) stem bark extract attenuates allergy and Freund&#039;s adjuvant-induced arthritis in rodentsPubmed:Pmc3996754
  • Nucleic Acids Res.MRTF-A steers an epigenetic complex to activate endothelin-induced pro-inflammatory transcription in vascular smooth muscle cellsPubmed:Pmc4176337
  • Int J Chron Obstruct Pulmon Dis. Increased serum levels of lipocalin-1 and-2 in patients with stable chronic obstructive pulmonary diseasePubmed:Pmc4043430
  • PLOS ONEMegakaryocytic Leukemia 1 (MKL1) Regulates Hypoxia Induced Pulmonary Hypertension in RatsPubmed:Pmc3960100
  • Inflamm Res. Intracranial injection of recombinant stromal-derived factor-1 alpha (SDF-1α) attenuates traumatic brain injury in ratsPubmed:24352531
  • InflammationEffects of honokiol on sepsis-induced acute kidney injury in an experimental model of sepsis in ratsPubmed:24531855
  • Biosens Bioelectron. A?new?signal?amplification?strategy?of?photoelectrochemical?immunoassay?for?highly?sensitive?interleukin-6?detection?based?on?TiO2/CdS/CdSe?dual?co-sensitized?structure.Pubmed:24690561
  • Immunology LettersEnhanced invasion of lung adenocarcinoma cells after co-culture with THP-1-derived macrophages via the induction of EMT by IL-6Pubmed:24698728
  • J Surg Res.Valproic acid protects septic mice from renal injury by reducing the inflammatory responsePubmed:24948542
  • Inflammation.?Alpha-lipoic acid exerts anti-inflammatory effects on lipopolysaccharide-stimulated rat mesangial cells via inhibition of nuclear factor kappa B (NF-κB) signaling pathway.Pubmed:24962643
  • Archives of Oral BiologyHyperlipidemia causes changes in inflammatory responses to periodontal pathogen challenge: Implications in acute and chronic infectionsPubmed:24992577
  • European Review for Medical and Pharmacological SciencesThe anti-inflammatory effects of 1,1 dimethyl-4- phenylpiperazinium (DMPP) compared to dexamethasone in a guinea pig model of ovalbumin induced asthmaPubmed:25070830
  • Inflammation.Oral administration?of?Saccharomyces?boulardii ameliorates carbon tetrachloride-induced liver fibrosis in?rats?via reducing intestinal permeability and modulating gut microbial composition.Pubmed:25227279
  • PLOS ONEBisphenol A Exposure Enhances Atherosclerosis in WHHL RabbitsPubmed:25333893
  • Journal of Dental ResearchNotch Signaling Induces Root Resorption via RANKL and IL-6 from hPDL CellsPubmed:25376720
  • Molecular Medicine ReportsGYY4137, a hydrogen sulfide?releasing molecule, inhibits the inflammatory response by suppressing the activation of nuclear factor?kappa B and mitogen?activated protein kinases in Coxsackie virus B3?infected rat cardiomyocytesPubmed:25377925
  • Life SciencesAdipose tissue regulates hepatic cholesterol metabolism via adiponectinPubmed:25445438
  • Journal of Surgical ResearchUrsolic acid improves survival and attenuates lung injury in septic rats induced by cecal ligation and puncturePubmed:25454976
  • Veterinary ResearchThe innate immune response of equine bronchial epithelial cells is altered by trainingPubmed:25595212
  • Journal of Translational MedicineProtective effects of astaxanthin against ischemia/reperfusion induced renal injury in micePubmed:25623758
  • Molecular Medicine ReportsTanshinone ⅡA attenuates bleomycin-induced pulmonary fibrosis in ratsPubmed:25672255
  • Reproduction in Domestic AnimalsThe Concentrations of Inflammatory Cytokines and Acute‐Phase Proteins in the Peripheral Blood and Uterine Washings in Cows with PyometraPubmed:25704413
  • Journal of Surgical ResearchExogenous carcinoembryonic antigen-related cell adhesion molecule 1 suppresses 2,4,6-trinitrobenzene sulfonic acid-induced ulcerative colitis in micePubmed:25724769
  •  International Journal of Clinical and Experimental PathologyPanax notoginseng saponins promote wound repair of anterior cruciate ligament through phosphorylation of PI3K, AKT and ERKPubmed:25755732
  • TheriogenologyInflammatory cytokines and acute-phase proteins concentrations in the peripheral blood and uterus of cows that developed endometritis during early postpartum.Pubmed:25765299
  • Journal of Surgical ResearchPivotal role of mast cell carboxypeptidase A in mediating protection against small intestinal ischemia–reperfusion injury in rats after ischemic preconditioningSciencedirect:S0022480414004909
  • Int J Clin Exp PatholRosiglitazone attenuates renal injury caused by hyperlipidemic pancreatitisPubMed: 26191125
  • Clinical and Experimental Pharmacology and PhysiologyCurcumin alleviates lung injury in diabetic rats by inhibiting nuclear factor‐κB pathwayPubMed: 26111829
  • Mediators of InflammationEffects of Intravenous Injection ofPubMed: 26063970
  • Int J Clin Exp PatholExpression of S100 family proteins in neonatal rats with sepsis and its significancePubMed: 25973048
  • Am J Physiol Heart Circ PhysiolIrisin improves endothelial function in obese mice through the AMPK-eNOS pathwayPubMed: 26371167
  • Biomedical ReportsAuricularia auricularBr: 3
  • Acta Vet ScandSerum IL-6 and IL-10 concentrations in bitches with pyometra undergoing ovariohysterectomyPubMed: 26410584
  • Clin Oral InvestigCan apical periodontitis affect serum levels of CRP, IL-2, and IL-6 as well as induce pathological changes in remote organs?PubMed: 26556576
  • Int J Clin Exp PatholEffects of dexmedetomidine on the protection of hyperoxia-induced lung injury in newborn ratsPubMed: 26261523
  • Front PharmacolGlycyrrhizic acid alleviates bleomycin-induced pulmonary fibrosis in ratsPubMed: 26483688
  • J Clin LipidolPlasma CTRP-3 concentrations in Chinese patients with obesity and type II diabetes negatively correlate with insulin resistancePubMed: 26073386
  • Cell Stress ChaperonesSalidroside protects against bleomycin-induced pulmonary fibrosis: activation of Nrf2-antioxidant signaling, and inhibition of NF-κB and TGF-β1/Smad-2/-3 pathwaysPubMed: 26577463
  • Life SciBetulin attenuates kidney injury in septic rats through inhibiting TLR4/NF-κB signaling pathwayPubMed: 26656467
  • Asian Pac J Trop MedEffect of SecinH3 on lung injury induced by sepsis of ratsPubMed: 26706678
  • PLOS ONEPenehyclidine Hydrochloride Preconditioning Provides Cardioprotection in a Rat Model of Myocardial Ischemia/Reperfusion InjuryPubMed: 26632817
  • PLOS ONEPlasma HMGB-1 Levels in Subjects with Obesity and Type 2 Diabetes: A Cross-Sectional Study in ChinaPubMed: 26317615
  • Int ImmunopharmacolBetulin attenuates lung and liver injuries in sepsisPubMed: 26644168
  • Journal of the Korean Society for Applied Biological ChemistryAnti-inflammatory effect ofArticle: 10.1007
  • J Histochem CytochemGlycyrrhizic Acid Prevents Sepsis-Induced Acute Lung Injury and Mortality in RatsPubMed: 26385569
  • Acta Cir BrasEffect of remote ischemic preconditioning in the expression of IL-6 and IL-10 in a rat model of liver ischemia-reperfusion injuryPubMed: 26270136
  • veterinary research forumEvaluation of proliferation and cytokines production by mitogen-stimulated bovine peripheral blood mononuclear cellsArticle_14936_6.Html:
  • Anim Reprod Sci.Alteration in peripheral blood concentration of certain pro-inflammatory cytokines in cows developing retention of fetal membranesPubMed: 25851495
  • Front Cell NeurosciPrenatal stress is a vulnerability factor for altered morphology and biological activity of microglia cellsPubMed: 25814933
  • Journal of Medicinal FoodA Standardized Extract ofPubMed: 26501382
  • Clin Exp Pharmacol PhysiolSilymarin attenuates paraquat-induced lung injury via Nrf2-mediated pathway in vivo and in vitroPubMed: 26173462
  • Int J Clin Exp MedBerberine protects vascular endothelial cells in hypertensive ratsPubMed: 26628971
  • Molecular Medicine ReportsEfficacy of coenzyme Q11 in mitigating spinal cord injuryPubMed: 26016719
  • Immunologic ResearchMesenchymal stromal cell-dependent reprogramming of Kupffer cells is mediated by TNF-α and PGE4 and is crucial for liver transplant tolerancePubMed: 25982496
  • Journal of Surgical ResearchCyanidin-3-O-glucoside attenuates acute lung injury in sepsis ratsPubMed: 26152793
  • Life SciencesNeuroprotective effect of allicin in a rat model of acute spinal cord injuryPubMed: 26546416
  • Tumor BiologyHypoxia-induced secretion of IL-18 from adipose-derived mesenchymal stem cell promotes growth and cancer stem cell properties of Burkitt lymphomaPubMed: 26695151
  • Biosens BioelectronUltrasensitive photoelectrochemical immunoassay for CA19-9 detection based on CdSe@ZnS quantum dots sensitized TiO2NWs/Au hybrid structure amplified by quenching effect of Ab2@V2+PubMed: 26433066
  • Hepatology ResearchAmelioration of free fatty acid-induced fatty liver by quercetin-3-O-尾-D-glucuronide through modulation of peroxisome proliferator-activated receptor-alpha/sterol regulatory element-binding protein-1c signalingPubMed: 26190035
  • Experimental and Therapeutic MedicineSignificance of the changes occurring in the levels of interleukins, SOD and MDA in rat pulmonary tissue following exposure to different altitudes and exposure times PubMed: 26622414
  • Медицина и здравоохранениеДИНАМИКА ЦИТОКИНОВОГО ПРОФИЛЯ В ОЦЕНКЕ ЭФФЕКТИВНОСТИ РАЗНЫХ МЕТОДОВ СТИМУЛЯЦИИ РЕПАРАТИВНОГО ОСТЕОГЕНЕЗА В ЭКСПЕРИМЕНТЕArticle: N
  • BMC AnesthesiologyHyperinflation deteriorates arterial oxygenation and lung injury in a rabbit model of ARDS with repeated open endotracheal suctioningPubMed: 25943099
  • J Reprod ImmunolThe immunomodulating effect of seminal plasma on T cellsPubMed: 25799173
  • International Journal of Advanced Research (2016Modulations of serum hepcidin, IL7 and iron status by different exercise regimens in ovarectomized ratsUploads: 605_Ijar-4848
  • TheriogenologyInflammatory cytokines and acute-phase proteins concentrations in the peripheral blood and uterus of cows that developed endometritis during early postpartumPubMed: 25765299
  • Vet Res CommunInflammatory cytokine and acute phase protein concentrations in the peripheral blood and uterine washings of cows with subclinical endometritis in the late postpartum periodPubMed: 25846950
  • Br J NutrEffects of prebiotic supplementation on the expression of proteins regulating iron absorption in anaemic growing ratsPubMed: 25745840
  • удкЦитокCgi-Bin: Irbis_Nbuv
  • удкАНАЛИЗ ЦИТОКИНОВОГО СТАТУСА ПРИ ОРВИ КРУПНОГО РОГАТОГО СКОТАUploads: Article
  • Oxidative Medicine and Cellular LongevityBQ123 Stimulates Skeletal Muscle Antioxidant Defense via Nrf2 Activation in LPS-Treated RatsJournals: Omcl
  • Immunol ResConstruction of recombinant Mip-FlaA dominant epitope vaccine against Legionella pneumophila and evaluation of the immunogenicity and protective immunityPubMed: 26607265